New chemotherapeutic drug sensitivity assay for colon carcinomas in monolayer culture Journal Article
| Authors: | Schroy, P. C. 3rd; Cohen, A.; Winawer, S. J.; Friedman, E. A. |
| Article Title: | New chemotherapeutic drug sensitivity assay for colon carcinomas in monolayer culture |
| Abstract: | Ten previously untreated colon carcinomas were tested for chemotherapeutic drug sensitivity in primary monolayer culture. Colon carcinomas were partly digested to groups of epithelial cells which plated with a mean efficiency of 42 ± 9% (SE) on a collagen I-bovine serum albumin substrate in serum-free medium, producing patches of tightly adherent epithelial cells. The cultured cells were judged epithelial by the presence of cytokeratins an epithelial cell surface epitope, junctional complexes, and brush borders. Each carcinoma was plated in 40 to 60 Petri dishes (35 mm), yielding a mean of 28 ± 8 (SE) colonies per dish (6832 ± 1952 cells). Drugs tested in duplicate plates were mitomycin C, cisplatin, streptozotocin, and 5-fluorouracil at 0.1, 1, 10, and 100 Mg/ml, and at 0.1,1, and 2x the peak tolerated drug concentration in serum. Twenty-four h after plating, any nonadherent cells were removed, and the adherent tumor cells were continuously exposed to the drugs for 3 days. Each drug induced colony lysis in a dose-dependent manner in responsive tumors. Drug-resistant, cycling cells were identified by [3H]thymidine incorporation in colonies which were not lysed by drug treatment. Each of the ten carcinomas exhibited inherent resistance to one or more chemotherapy drugs within the concentration ranges clinically achievable. © 1988, American Association for Cancer Research. All rights reserved. |
| Keywords: | human cell; cisplatin; fluorouracil; antineoplastic agents; methodology; cytology; colonic neoplasms; drug resistance; drug screening assays, antitumor; tumor cells, cultured; assay; histology; cell culture; mitomycin c; colon carcinoma; mitomycin; drug sensitivity; ultrastructure; streptozocin; tissue culture; mitomycins; human; support, non-u.s. gov't; support, u.s. gov't, p.h.s. |
| Journal Title: | Cancer Research |
| Volume: | 48 |
| Issue: | 11 |
| ISSN: | 0008-5472 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 1988-06-01 |
| Start Page: | 3236 |
| End Page: | 3244 |
| Language: | English |
| PUBMED: | 2966672 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 6 August 2020 -- Source: Scopus |
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