| Abstract: |
Purpose: To determine if increased perfusion using dynamic susceptibility contrast perfusion MRI (DSC MRI) in gliomas may be predictive of 1p19q deletions. Loss of heterozygosity of chromosomes 1p and 19q confers responsiveness to chemotherapy improving survival in gliomas. Materials and Methods: We retrospectively reviewed 16 patients who had DSC MRI and molecular studies of their excised gliomas for 1p19q deletions. Allelic status was assessed by loss of heterozygosity using polymerase chain reaction (PCR). DNA was extracted from paraffin curls of brain tumor sections and nail clippings. Relative cerebral blood volume (rCBV) measurements were then statistically compared with the presence of 1p and 19q deletions. Results: Patients with 1p19q deletions (N = 7) demonstrated rCBV values of 10.54 ± 2.93. Patients without 1p deletions (N = 9) had rCBV values of 4.84 ± 2.4 (P = 0.012). Logistic regression demonstrated that rCBV was able to predict the presence of a 1p deletion to significance levels of 0.038 and 0.044, adjusted and not adjusted for age and sex, respectively. The kappa coefficient for the agreement between predicted deletion status using rCBV and the truedeletion status was 0.746 (P = 0.0028). Deletions of 19q alone, or together with 1p deletions, were not associated with high rCBV. Conclusion: Histopathologic, molecular, and imaging evidence supports increased neovascularity in gliomas with 1p deletions in this preliminary study. We propose a diagnostic algorithm to obtain molecular studies in gliomas demonstrating high rCBV. © 2007 Wiley-Liss, Inc. |
| Keywords: |
adult; cancer survival; clinical article; human tissue; aged; aged, 80 and over; middle aged; retrospective studies; histopathology; nuclear magnetic resonance imaging; glioma; brain neoplasms; magnetic resonance imaging; antineoplastic agent; polymerase chain reaction; chromosome; allele; logistic models; retrospective study; algorithms; in situ hybridization; gene expression regulation, neoplastic; algorithm; contrast enhancement; predictive value of tests; chromosomes, human, pair 19; contrast media; heterozygosity loss; loss of heterozygosity; chromosome deletion; chromosomes, human, pair 1; blood volume; dna extraction; brain blood volume; cerebrovascular circulation; statistics, nonparametric; genetic markers; chromosome 1p; paraffin; perfusion; chromosome 19q; molecular deletions; neovascularity; perfusion mri
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