A mammalian microRNA expression atlas based on small RNA library sequencing Journal Article
| Authors: | Landgraf, P.; Rusu, M.; Sheridan, R.; Sewer, A.; Iovino, N.; Aravin, A.; Pfeffer, S.; Rice, A.; Kamphorst, A. O.; Landthaler, M.; Lin, C.; Socci, N. D.; Hermida, L.; Fulci, V.; Chiaretti, S.; Foà, R.; Schliwka, J.; Fuchs, U.; Novosel, A.; Müller, R. U.; Schermer, B.; Bissels, U.; Inman, J.; Phan, Q.; Chien, M.; Weir, D. B.; Choksi, R.; De Vita, G.; Frezzetti, D.; Trompeter, H. I.; Hornung, V.; Teng, G.; Hartmann, G.; Palkovits, M.; Di Lauro, R.; Wernet, P.; Macino, G.; Rogler, C. E.; Nagle, J. W.; Ju, J.; Papavasiliou, F. N.; Benzing, T.; Lichter, P.; Tam, W.; Brownstein, M. J.; Bosio, A.; Borkhardt, A.; Russo, J. J.; Sander, C.; Zavolan, M.; Tuschl, T. |
| Article Title: | A mammalian microRNA expression atlas based on small RNA library sequencing |
| Abstract: | MicroRNAs (miRNAs) are small noncoding regulatory RNAs that reduce stability and/or translation of fully or partially sequence-complementary target mRNAs. In order to identify miRNAs and to assess their expression patterns, we sequenced over 250 small RNA libraries from 26 different organ systems and cell types of human and rodents that were enriched in neuronal as well as normal and malignant hematopoietic cells and tissues. We present expression profiles derived from clone count data and provide computational tools for their analysis. Unexpectedly, a relatively small set of miRNAs, many of which are ubiquitously expressed, account for most of the differences in miRNA profiles between cell lineages and tissues. This broad survey also provides detailed and accurate information about mature sequences, precursors, genome locations, maturation processes, inferred transcriptional units, and conservation patterns. We also propose a subclassification scheme for miRNAs for assisting future experimental and computational functional analyses. © 2007 Elsevier Inc. All rights reserved. |
| Keywords: | controlled study; human tissue; gene sequence; nonhuman; mammalia; animals; mice; animal tissue; microrna; gene expression profiling; cell maturation; genetic variability; gene library; cell lineage; cell type; rna; gene expression regulation; hematologic neoplasms; conserved sequence; molecular sequence data; rna, messenger; mammal; cancer cell; rat; hematopoietic system; hematopoietic stem cells; base sequence; rats; rodent; rodentia; micrornas; sequence homology, nucleic acid; molimmuno; gene location; phylogeny; maturation; moleneuro |
| Journal Title: | Cell |
| Volume: | 129 |
| Issue: | 7 |
| ISSN: | 0092-8674 |
| Publisher: | Cell Press |
| Date Published: | 2007-06-29 |
| Start Page: | 1401 |
| End Page: | 1414 |
| Language: | English |
| DOI: | 10.1016/j.cell.2007.04.040 |
| PUBMED: | 17604727 |
| PROVIDER: | scopus |
| PMCID: | PMC2681231 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 717" - "Export Date: 17 November 2011" - "CODEN: CELLB" - "Source: Scopus" |
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