Measurement of terminal deoxynucleotidyl transferase mRNA in clinical samples: A new parameter in analysis of leukemia cells Journal Article
| Authors: | Wolf, S. C.; Steinherz, P. G.; Landau, N. R.; Silverstone, A. E. |
| Article Title: | Measurement of terminal deoxynucleotidyl transferase mRNA in clinical samples: A new parameter in analysis of leukemia cells |
| Abstract: | A 1750 base pair cDNA to human terminal deoxynucleotidyl transferase (TdT) has been cloned. This cDNA detects a dominant 2200 base pair messenger RNA species in normal and leukemic cells synthesizing the enzyme. A quantitative dot blot assay was utilized to survey a number of clinical samples from patients with TdT positive and negative leukemias as well as cells from normal volunteers. A linear relationship was detected between the amount of TdT mRNA and the amount of enzyme activity in bone marrow cells. The assay is sensitive enough to detect normal TdT levels in bone marrow, and distinguish these levels from the lack of such mRNA in peripheral blood and bone marrow of patients with myeloid leukemia. Elevated levels of mRNA were found in two cases of patients in clinical remission. The prognostic significance of these observations must await further study. Copyright © 1987 Wiley‐Liss, Inc., A Wiley Company |
| Keywords: | leukemia; bone marrow cells; bone marrow; assay; dna; rna, messenger; leukemia cell; dna nucleotidylexotransferase; clone cells; complementary dna; electrophoresis, polyacrylamide gel; dna nucleotidyltransferases; cdna; base composition; leukemia, myelocytic, acute; leukemia, lymphocytic; precipitin tests; human; priority journal; collodion; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; translation, genetic; tdt |
| Journal Title: | American Journal of Hematology |
| Volume: | 25 |
| Issue: | 3 |
| ISSN: | 0361-8609 |
| Publisher: | John Wiley & Sons, Inc. |
| Date Published: | 1987-07-01 |
| Start Page: | 259 |
| End Page: | 269 |
| Language: | English |
| DOI: | 10.1002/ajh.2830250305 |
| PUBMED: | 3474890 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 5 February 2021 -- Source: Scopus; Acknowledgements: We wish to thank Jeni Mum for technical assistance and June Connolly for typing this manuscript. |
