Anti gC1qR/p32/HABP1 antibody therapy decreases tumor growth in an orthotopic murine xenotransplant model of triple negative breast cancer Journal Article

   

Authors:	Peerschke, E. I.; de Stanchina, E.; Chang, Q.; Manova-Todorova, K.; Barlas, A.; Savitt, A. G.; Geisbrecht, B. V.; Ghebrehiwet, B.
Article Title:	Anti gC1qR/p32/HABP1 antibody therapy decreases tumor growth in an orthotopic murine xenotransplant model of triple negative breast cancer
Abstract:	gC1qR is highly expressed in breast cancer and plays a role in cancer cell proliferation. This study explored therapy with gC1qR monoclonal antibody 60.11, directed against the C1q binding domain of gC1qR, in a murine orthotopic xenotransplant model of triple negative breast cancer. MDA231 breast cancer cells were injected into the mammary fat pad of athymic nu/nu female mice. Mice were segregated into three groups (n = 5, each) and treated with the vehicle (group 1) or gC1qR antibody 60.11 (100 mg/kg) twice weekly, starting at day 3 post-implantation (group 2) or when the tumor volume reached 100 mm(3) (group 3). At study termination (d = 35), the average tumor volume in the control group measured 895 +/- 143 mm(3), compared to 401 +/- 48 mm(3) and 701 +/- 100 mm(3) in groups 2 and 3, respectively (p < 0.05). Immunohistochemical staining of excised tumors revealed increased apoptosis (caspase 3 and TUNEL staining) in 60.11-treated mice compared to controls, and decreased angiogenesis (CD31 staining). Slightly decreased white blood cell counts were noted in 60.11-treated mice. Otherwise, no overt toxicities were observed. These data are the first to demonstrate an in vivo anti-tumor effect of 60.11 therapy in a mouse model of triple negative breast cancer.
Keywords:	breast cancer; receptor; proliferation; expression; identification; overexpression; cell-surface; gc1qr; globular heads; c1q; hyaluronan-binding protein; gc1q-r; xenotransplant model
Journal Title:	Antibodies
Volume:	9
Issue:	4
ISSN:	2073-4468
Publisher:	MDPI
Date Published:	2020-12-01
Start Page:	51
Language:	English
ACCESSION:	WOS:000601451000001
DOI:	10.3390/antib9040051
PROVIDER:	wos
PMCID:	PMC7709104
PUBMED:	33036212
Notes:	Article -- Source: Wos

https://synapse.mskcc.org/synapse/works/has_related_data_chk/176248