Recombination signatures distinguish embryonic stem cells derived by parthenogenesis and somatic cell nuclear transfer Journal Article
| Authors: | Kim, K.; Ng, K.; Rugg-Gunn, P. J.; Shieh, J.H.; Kirak, O.; Jaenisch, R.; Wakayama, T.; Moore, M. A.; Pedersen, R. A.; Daley, G. Q. |
| Article Title: | Recombination signatures distinguish embryonic stem cells derived by parthenogenesis and somatic cell nuclear transfer |
| Abstract: | Parthenogenesis and somatic cell nuclear transfer (SCNT) are two methods for deriving embryonic stem (ES) cells that are genetically matched to the oocyte donor or somatic cell donor, respectively. Using genome-wide single nucleotide polymorphism (SNP) analysis, we demonstrate distinct signatures of genetic recombination that distinguish parthenogenetic ES cells from those generated by SCNT. We applied SNP analysis to the human ES cell line SCNT-hES-1, previously claimed to have been derived by SCNT, and present evidence that it represents a human parthenogenetic ES cell line. Genome-wide SNP analysis represents a means to validate the genetic provenance of an ES cell line. © 2007 Elsevier Inc. All rights reserved. |
| Keywords: | controlled study; human cell; single nucleotide polymorphism; polymorphism, single nucleotide; nonhuman; validation process; animal cell; animals; mice; somatic cell; embryonic stem cell; cell line; genotype; heterozygote; dna methylation; genetic recombination; specimen handling; recombination, genetic; human genome; stemcell; homozygote; embryonic stem cells; cytogenetic analysis; genome, human; sequence analysis, dna; cell nucleus transplantation; parthenogenesis; nuclear transfer techniques |
| Journal Title: | Cell Stem Cell |
| Volume: | 1 |
| Issue: | 3 |
| ISSN: | 1934-5909 |
| Publisher: | Cell Press |
| Date Published: | 2007-09-13 |
| Start Page: | 346 |
| End Page: | 352 |
| Language: | English |
| DOI: | 10.1016/j.stem.2007.07.001 |
| PUBMED: | 18371368 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 53" - "Export Date: 17 November 2011" - "Source: Scopus" |
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