A Polypodium leucotomos extract inhibits solar-simulated radiation-induced TNF-α and iNOS expression, transcriptional activation and apoptosis Journal Article
| Authors: | Jańczyk, A.; Garcia-Lopez, M. A.; Fernandez-Peñas, P.; Alonso-Lebrero, J. L.; Benedicto, I.; López-Cabrera, M.; Gonzalez, S. |
| Article Title: | A Polypodium leucotomos extract inhibits solar-simulated radiation-induced TNF-α and iNOS expression, transcriptional activation and apoptosis |
| Abstract: | In this report, we have examined the molecular basis of the photoprotective effect of a hydrophilic extract of the fern Polypodium leucotomos (PL) in vitro, using a solar simulator as the source of UV radiation (SSR). We found that pretreatment of human keratinocytes with PL inhibited SSR-mediated increase of tumor necrosis factor (TNF)-α and also abrogated nitric oxide (NO) production. Consistent with this, PL blocked the induction of inducible nitric oxide synthase (iNOS) elicited by SSR. In addition, PL inhibited the SSR-mediated transcriptional activation of NF-κB and AP1. Finally, we demonstrated that pretreatment with PL exerted a cytoprotective effect against SSR-induced damage, resulting in increased cell survival. Together, these data postulate a multifactor mechanism of protection not exclusively reliant on the antioxidant capability of PL, and strengthen the basic knowledge on the photoprotective effect of this botanical agent. © 2007 The Authors Journal compilation © 2007 Blackwell Munksgaard. |
| Keywords: | controlled study; human cell; ultraviolet radiation; cell viability; cell survival; apoptosis; enzyme inhibition; gene expression; molecular dynamics; cell line; immunoglobulin enhancer binding protein; transcription, genetic; dose-response relationship, drug; keratinocyte; simulation; transcription regulation; tumor necrosis factor alpha; tumor necrosis factor-alpha; cell damage; nf-kappa b; plant extracts; ascorbic acid; antioxidant activity; ultraviolet rays; luciferases; cell protection; keratinocytes; sunlight; sunlight protection; inducible nitric oxide synthase; nitric oxide synthase type ii; polypodium leucotomos extract; polypodium; tnf-alpha; nitric oxide; transcription factor ap 1; photoprotection; transcription factor ap-1; plant leaves; hacat; inos |
| Journal Title: | Experimental Dermatology |
| Volume: | 16 |
| Issue: | 10 |
| ISSN: | 0906-6705 |
| Publisher: | Wiley Blackwell |
| Date Published: | 2007-10-01 |
| Start Page: | 823 |
| End Page: | 829 |
| Language: | English |
| DOI: | 10.1111/j.1600-0625.2007.00603.x |
| PUBMED: | 17845214 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 16" - "Export Date: 17 November 2011" - "CODEN: EXDEE" - "Source: Scopus" |
