Genome-wide dynamics of SAPHIRE, an essential complex for gene activation and chromatin boundaries Journal Article
| Authors: | Gordon, M.; Holt, D. G.; Panigrahi, A.; Wilhelm, B. T.; Erdjument-Bromage, H.; Tempst, P.; Bähler, J.; Cairns, B. R. |
| Article Title: | Genome-wide dynamics of SAPHIRE, an essential complex for gene activation and chromatin boundaries |
| Abstract: | In this study, we characterize a four-protein nucleosome-binding complex from Schizosaccharomyces pombe, termed SAPHIRE, that includes two orthologs of human Lsd1, a histone demethylase. The SAPHIRE complex is essential for cell viability, whereas saphire mutants lacking key conserved catalytic residues are viable but thermosensitive, suggesting that SAPHIRE has both an important enzymatic function and an essential nonenzymatic function. SAPHIRE is present in (or adjacent to) particular heterochromatic loci and also in the transcription start site regions of many highly active polymerase II genes. However, ribosomal protein genes are notably SAPHIRE deficient. SAPHIRE promotes activation, as target genes are selectively attenuated in saphire mutants. Interestingly, saphire mutants display increased histone H3 lysine 4 dimethylation, a modification typically associated with euchromatin. SAPHIRE localization is dynamic, as activated genes rapidly acquire SAPHIRE. Furthermore, saphire mutants dramatically shift a heterochromatin-euchromatin boundary in Chr1, suggesting a novel role in boundary regulation. Copyright © 2007, American Society for Microbiology. All Rights Reserved. |
| Keywords: | nonhuman; mutant protein; protein localization; telomere; animals; cell viability; cell survival; molecular dynamics; protein binding; genetic transcription; enzyme activity; gene expression regulation; genome analysis; gene activation; amino acid sequence; molecular sequence data; sequence alignment; chromatin; multiprotein complexes; catalysis; protein subunits; heat-shock response; histones; heterochromatin; schizosaccharomyces; schizosaccharomyces pombe proteins; ribosome protein; chromatin structure; transcription initiation site; schizosaccharomyces pombe protein; schizosaccharomyces pombe; genome, fungal; nucleosomes; dna directed dna polymerase alpha; temperature sensitive mutant; oxidoreductases, n-demethylating |
| Journal Title: | Molecular and Cellular Biology |
| Volume: | 27 |
| Issue: | 11 |
| ISSN: | 0270-7306 |
| Publisher: | American Society for Microbiology |
| Date Published: | 2007-06-01 |
| Start Page: | 4058 |
| End Page: | 4069 |
| Language: | English |
| DOI: | 10.1128/mcb.02044-06 |
| PUBMED: | 17371846 |
| PROVIDER: | scopus |
| PMCID: | PMC1900013 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 9" - "Export Date: 17 November 2011" - "CODEN: MCEBD" - "Source: Scopus" |
