SET and PARP1 remove DEK from chromatin to permit access by the transcription machinery Journal Article
| Authors: | Gamble, M. J.; Fisher, R. P. |
| Article Title: | SET and PARP1 remove DEK from chromatin to permit access by the transcription machinery |
| Abstract: | The histone chaperone SET is required for transcription of chromatin templates by RNA polymerase Pol II (Pol II) in vitro. Here we uncover a positive role for SET in dislodging DEK and PARP1, which restrict access to chromatin in the absence of SET and the PARP1 substrate NAD+. SET binds chromatin, dissociating DEK and PARP1 to allow transcription in the absence of NAD+. In the absence of SET, depletion of DEK restores chromatin accessibility to endonuclease but does not permit Mediator recruitment or transcription. In the presence of NAD+, PARP1 poly(ADP-ribosyl)ates and evicts DEK (and itself) from chromatin to permit Mediator loading and transcription independent of SET. An artificial DEK variant resistant to SET and PARP1 represses transcription, indicating a requirement for DEK removal. Therefore, SET, DEK and PARP1 constitute a network governing access to chromatin by the transcription machinery. © 2007 Nature Publishing Group. |
| Keywords: | controlled study; unclassified drug; human cell; protein function; chromosomal proteins, non-histone; protein protein interaction; protein; genetic transcription; protein interaction; transcription, genetic; hela cells; transcription factors; transcription regulation; chromatin; oncogene proteins; recombinant protein; molecular interaction; endonuclease; rna polymerase ii; nicotinamide adenine dinucleotide; functional proteomics; poly(adp-ribose) polymerases; nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase 1; protein dek; protein set |
| Journal Title: | Nature Structural and Molecular Biology |
| Volume: | 14 |
| Issue: | 6 |
| ISSN: | 1545-9993 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2007-06-01 |
| Start Page: | 548 |
| End Page: | 555 |
| Language: | English |
| DOI: | 10.1038/nsmb1248 |
| PUBMED: | 17529993 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 28" - "Export Date: 17 November 2011" - "CODEN: NSMBC" - "Source: Scopus" |
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