| Abstract: |
Pro-brain-derived neurotrophic factor (proBDNF) and mature BDNF utilize distinct receptors to mediate divergent neuronal actions. Using new tools to quantitate endogenous BDNF isoforms, we found that mouse neurons secrete both proBDNF and mature BDNF. The highest levels of proBDNF and p75 were observed perinatally and declined, but were still detectable, in adulthood. Thus, BDNF actions are developmentally regulated by secretion of proBDNF or mature BDNF and by local expression of p75 and TrkB. © 2009 Nature America, Inc. All rights reserved. |
| Keywords: |
protein expression; nonhuman; protein function; animal cell; mouse; animals; mice; cell survival; cells, cultured; mice, mutant strains; neurons; gene expression regulation, developmental; antibodies, monoclonal; protein synthesis; quantitative analysis; brain derived neurotrophic factor; brain-derived neurotrophic factor; protein secretion; gene knock-in techniques; nerve cell; protein variant; protein determination; isoprotein; brain derived neurotrophic factor receptor; protein p75; perinatal period; protein synthesis regulation; hippocampus; protein precursors; receptor, nerve growth factor; receptor, trkb
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