Autonomous growth potential of leukemia blast cells is associated with poor prognosis in human acute leukemias Journal Article
| Authors: | Yan, Y.; Wieman, E. A.; Guan, X.; Jakubowski, A. A.; Steinherz, P. G.; O'Reilly, R. J. |
| Article Title: | Autonomous growth potential of leukemia blast cells is associated with poor prognosis in human acute leukemias |
| Abstract: | We have described a severe combined immunodeficiency (SCID) mouse model that permits the subcutaneous growth of primary human acute leukemia blast cells into a measurable subcutaneous nodule which may be followed by the development of disseminated disease. Utilizing the SCID mouse model, we examined the growth potential of leukemic blasts from 133 patients with acute leukemia, (67 acute lymphoblastic leukemia (ALL) and 66 acute myeloid leukemia (AML)) in the animals after subcutaneous inoculation without conditioning treatment. The blasts displayed three distinct growth patterns: "aggressive", "indolent", or "no tumor growth". Out of 133 leukemias, 45 (33.8%) displayed an aggressive growth pattern, 14 (10.5%) displayed an indolent growth pattern and 74 (55.6%) did not grow in SCID mice. The growth probability of leukemias from relapsed and/or refractory disease was nearly 3 fold higher than that from patients with newly diagnosed disease. Serial observations found that leukemic blasts from the same individual, which did not initiate tumor growth at initial presentation and/or at early relapse, may engraft and grow in the later stages of disease, suggesting that the ability of leukemia cells for engraftment and proliferation was gradually acquired following the process of leukemia progression. Nine autonomous growing leukemia cell lines were established in vitro. These displayed an aggressive proliferation pattern, suggesting a possible correlation between the capacity of human leukemia cells for autonomous proliferation in vitro and an aggressive growth potential in SCID mice. In addition, we demonstrated that patients whose leukemic blasts displayed an aggressive growth and dissemination pattern in SClD mice had a poor clinical outcome in patients with ALL as well as AML. Patients whose leukemic blasts grew indolently or whose leukemia cells failed to induce growth had a significantly longer DFS and more favorable clinical course. © 2009 Yan et al; licensee BioMed Central Ltd. |
| Keywords: | adolescent; adult; child; controlled study; preschool child; school child; aged; child, preschool; middle aged; leukemia; young adult; acute granulocytic leukemia; major clinical study; disease course; mortality; cancer growth; disseminated cancer; nonhuman; cancer staging; cancer diagnosis; cell proliferation; mouse; animal; mouse mutant; animals; mice; animal tissue; animal experiment; animal model; cancer cell culture; in vitro study; pathology; mice, scid; acute lymphoblastic leukemia; acute leukemia; engraftment; xenograft; evaluation; infant; leukemia cell; prediction and forecasting; predictive value of tests; transplantation, heterologous; cancer relapse; neoplasm transplantation; lymphocyte; lymphocytes; severe combined immunodeficiency; inoculation; acute disease; refractory period; cancer transplantation; aggression; blast cell; subcutaneous nodule |
| Journal Title: | Journal of Hematology & Oncology |
| Volume: | 2 |
| ISSN: | 1756-8722 |
| Publisher: | Biomed Central Ltd |
| Date Published: | 2009-01-01 |
| Start Page: | 51 |
| Language: | English |
| DOI: | 10.1186/1756-8722-2-51 |
| PUBMED: | 20040095 |
| PROVIDER: | scopus |
| PMCID: | PMC2807866 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 30 November 2010" - "Art. No.: 51" - "Source: Scopus" |
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