The Ly-10 antigen is a marker of mouse-activated T lymphocytes Journal Article
| Authors: | Chan, M. M.; Hämmerling, U.; Stutman, O. |
| Article Title: | The Ly-10 antigen is a marker of mouse-activated T lymphocytes |
| Abstract: | Ly-10.1 is a lymphocyte surface antigen controlled by a gene linked to the Ly-1.1 locus and expressed on activated T helper, T suppressor (Ts), and cytotoxic T lymphocytes (CTL). In this report, we describe the following: 1) Ly-10 is a heterodimeric glycoprotein consisiting of a 80 000 heavy and a 34 000 light chain. 2) Although mature CTL are Ly-10+ by negative selection with anti-Ly-10.1 and complement (C), CTL precursors reactive to allogeneic cells are Ly-10-. 3) Similarly, IL-2-producing effector T cells induced by MIs-incompatible cells and semiallogeneic stimulation are eliminated by anti-Ly-10.1 and C after activation but are not eliminated as precursors before activation. 4) In mixed lymphocyte culture with semiallogeneic cells, the frequency of Ly-10.1+ cells was highest on the 2nd to 5th day after stimulation, decreased by the 12th day, and increased after restimulation with fresh antigen as demonstrated by immunofluorescence, C-mediated lysis, and IL-2 production. 5) When spleen cells were treated with anti-Ly-10 and C before concanavalin A (Con A) activation, the suppressive activity in the Con A T blasts was reduced, suggesting that in normal mice, some Ts preexist in a Ly-10+ activated state. These results indicate that Ly-10 is a marker of activation of T cells, not expressed on precursor T cells and whose expression is both transient and dependent on the presence of antigen. The similarities in biochemical and cellular characteristics suggest that Ly-10 is a mouse homologue of the human lymphocyte activation marker 4F2. © 1988 Springer-Verlag. |
| Keywords: | nonhuman; animal cell; mouse; phenotype; animal; mice; immunofluorescence; mice, inbred balb c; mice, inbred c57bl; fluorescent antibody technique; membrane antigen; lymphocyte activation; lymphocyte culture test, mixed; kinetics; cell culture; t-lymphocytes, cytotoxic; stem cells; mice, inbred cba; biochemistry; t lymphocyte activation; antigens, ly; ly antigen; mixed lymphocyte reaction; interleukin-2; antigens, differentiation, t-lymphocyte; concanavalin a; priority journal; support, u.s. gov't, p.h.s.; t-lymphocytes, suppressor-effector |
| Journal Title: | Immunogenetics |
| Volume: | 28 |
| Issue: | 6 |
| ISSN: | 0093-7711 |
| Publisher: | Springer |
| Date Published: | 1988-12-01 |
| Start Page: | 425 |
| End Page: | 432 |
| Language: | English |
| DOI: | 10.1007/bf00355374 |
| PUBMED: | 2972612 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Source: Scopus |
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