Ly‐6 A/E antigen of murine T cells is associated with a distinct pathway of activation. Requirements for interferon and exogenous interleukin 2 Journal Article

  


Authors: Toulon, M.; Palfree, R. G. E.; Palfree, S.; Dumont, F. J.; Hämmerling, U.
Article Title: Ly‐6 A/E antigen of murine T cells is associated with a distinct pathway of activation. Requirements for interferon and exogenous interleukin 2
Abstract: The Ly‐6 pathway of T cell activation was analyzed to identify its essential requirements. Using a monospecific chicken antiserum to Ly‐6E, fully cross‐reactive to its allelic counterpart, Ly‐6A, but unreactive with other members of the Ly‐6 family, we have found that interferon (IFN)‐α/β or γ, Ly‐6 antibody and interleukin 2 (IL2) act synergistically in inducing T cell proliferation. The action of IFN can be attributed to induction of Ly‐6 A/E antigen on T cells, as described previously, and this induction is transcriptionally controlled. Exposure of T cells with elevated Ly‐6 concentrations to chicken anti‐Ly‐6 antibody leads to expression of IL2 receptors. Consequently, the addition of IL2 drives T cell proliferation. Thus, in BALB/c mice the minimum requirements for activation by the Ly‐6 pathway are IFN (as a means of inducing Ly‐6), Ly‐6 antibody (as inducer of IL2 receptors) and IL2. In mice of the Ly‐6. 2 haplotype, IFN is not an absolute requirement. This may be related to the fact that these animals, in contrast to those of Ly‐6.1 haplotype, express Ly‐6 constitutively on a substantial proportion of resting T cells. Thus, T cells of C57BL/6 or DBA/2 mice can be induced to proliferate with Ly‐6 antibody and IL2 alone, although IFN pre‐treatment enhances this response. In BALBic mice the IL2‐driven proliferative response induced through the Ly‐6 pathway occurs selectively in the L3T4− population. Copyright © 1988 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
Keywords: interferon; nonhuman; lymphocyte proliferation; t-lymphocytes; animal cell; mouse; phenotype; animal; mice; interleukin 2; mice, inbred balb c; mice, inbred c57bl; lymphocyte activation; genetic engineering; cell culture; antibody specificity; t lymphocyte activation; antigens, ly; interleukin-2; receptors, immunologic; chickens; antigen-antibody reactions; antigens, differentiation, t-lymphocyte; receptors, interleukin-2; immune sera; mice, inbred dba; interferons; male; female; support, u.s. gov't, p.h.s.
Journal Title: European Journal of Immunology
Volume: 18
Issue: 6
ISSN: 0014-2980
Publisher: Wiley V C H Verlag Gmbh  
Date Published: 1988-06-01
Start Page: 937
End Page: 942
Language: English
DOI: 10.1002/eji.1830180616
PUBMED: 2454826
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0023936115&doi=10.1002%2feji.1830180616&partnerID=40&md5=7bf1e3787d1576459111f7ab6026fc3a
Notes: Article -- Export Date: 6 August 2020 -- Source: Scopus
Altmetric
What is Altmetric?
Citation Impact
What is Dimensions Citation Badge?
BMJ Impact Analytics
MSK Authors
  1. Ulrich Hammerling
    79 Hammerling
  2. Roger G. E. Palfree
    17 Palfree
Related MSK Work