Intra-H-2 recombination in t haplotypes shows a hot spot and close linkage of 1(tw5) to H-2K Journal Article
| Authors: | Artzt, K.; Abe, K.; Uehara, H.; Bennett, D. |
| Article Title: | Intra-H-2 recombination in t haplotypes shows a hot spot and close linkage of 1(tw5) to H-2K |
| Abstract: | The embryonic lethal mutation in the tw5 haplotype is known to map near the H-2K region of the mouse major histocompatibility complex. Additional data obtained by classical genetic methods demonstrate that the tw5 lethal gene is effectively inseparable from H-2K. No recombinants were found between H-2K and tw5 in a sample representing over 1200 mice. On a statistical basis tw5 must be less than 250 kb from the H-2K gene. In the course of these mapping studies we obtained a set of 11 intra-H-2 recombinants. We have analyzed these and three others derived from another experiment to define their breakpoints as precisely as possible. Southern blot analysis with molecular probes to the D, S, I, and K regions of the H-2 complex defines seven recombinations between the D and S regions, two between S and I, none within the I region, and five events between I and K. The last category was studied in finer detail by developing unique copy probes to the I-K boundary region. Two of the five events occurred within probably less than 6 kb of each other: these two recombinants define the centromeric limit of the location of the tw5 gene within the H-2K region. The other three I-K recombinants occurred in at least two other nearby locations. Altogether at least three, and probably all five I-K recombinants fall within a 45 kb recombinational hot spot recently identified in Mus musculus castaneus. © 1988 Springer-Verlag. |
| Keywords: | nonhuman; animal cell; mouse; animal; mice; animal experiment; mice, mutant strains; haplotype; genetic recombination; genetic engineering; cell culture; recombination, genetic; major histocompatibility complex; linkage (genetics); genetic linkage; chromosome mapping; genes, lethal; mice, inbred c3h; h-2 antigens; female; priority journal; support, u.s. gov't, p.h.s. |
| Journal Title: | Immunogenetics |
| Volume: | 28 |
| Issue: | 1 |
| ISSN: | 0093-7711 |
| Publisher: | Springer |
| Date Published: | 1988-07-01 |
| Start Page: | 30 |
| End Page: | 37 |
| Language: | English |
| DOI: | 10.1007/bf00372526 |
| PUBMED: | 3378835 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Source: Scopus |
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