Dietary lutein/zeaxanthin partially reduces photoaging and photocarcinogenesis in chronically UVB-irradiated Skh-1 hairless mice Journal Article


Authors: Astner, S.; Wu, A.; Chen, J.; Philips, N.; Rius-Diaz, F.; Parrado, C.; Mihm, M. C.; Goukassian, D. A.; Pathak, M. A.; Gonzalez, S.
Article Title: Dietary lutein/zeaxanthin partially reduces photoaging and photocarcinogenesis in chronically UVB-irradiated Skh-1 hairless mice
Abstract: Lutein and zeaxanthin are xanthophyll carotenoids with potent antioxidant properties protecting the skin from acute photodamage. This study extended the investigation to chronic photodamage and photocarcinogenesis. Mice received either a lutein/zeaxanthin-supplemented diet or a standard nonsupplemented diet. Dorsal skin of female Skh-1 hairless mice was exposed to UVB radiation with a cumulative dose of 16,000 mJ/cm2 for photoaging and 30,200 mJ/cm 2 for photocarcinogenesis. Clinical evaluations were performed weekly, and the animals were sacrificed 24 h after the last UVB exposure. For photoaging experiments, skin fold thickness, suprapapillary plate thickness, mast cell counts and dermal desmosine content were evaluated. For photocarcinogenesis, samples of tumors larger than 2 mm were analyzed for histological characterization, hyperproliferation index, tumor multiplicity, total tumor volume and tumor-free survival time. Results of the photoaging experiment revealed that skin fold thickness and number of infiltrating mast cells following UVB irradiation were significantly less in lutein/zeaxanthin- treated mice when compared to irradiated animals fed the standard diet. The results of the photocarcinogenesis experiment were increased tumor-free survival time, reduced tumor multiplicity and total tumor volume in lutein/zeaxanthin- treated mice in comparison with control irradiated animals fed the standard diet. These data demonstrate that dietary lutein/zeaxanthin supplementation protects the skin against UVB-induced photoaging and photocarcinogenesis. Copyright © 2007 S. Karger AG.
Keywords: controlled study; unclassified drug; histopathology; nonhuman; disease free survival; radiation dose; animal cell; mouse; animals; mice; animal tissue; skin neoplasms; cell infiltration; tumor volume; animal experiment; animal model; skin cancer; diet supplementation; radiation exposure; skin; standard; survival time; diet; tumor burden; cell count; diet therapy; xanthophyll; ultraviolet b radiation; ultraviolet rays; skin carcinogenesis; photoaging; ultraviolet irradiation; skin aging; hairless mouse; mast cell; mast cells; skinfold thickness; amino acid composition; photocarcinogenesis; light damage; carotenoids; lutein; zeaxanthin; desmosine; floraglo; xanthophyll plus zeaxanthin; mice, inbred hrs; xanthophylls
Journal Title: Skin Pharmacology and Physiology
Volume: 20
Issue: 6
ISSN: 1660-5527
Publisher: S. Karger AG  
Date Published: 2007-10-01
Start Page: 283
End Page: 291
Language: English
DOI: 10.1159/000107576
PUBMED: 17717424
PROVIDER: scopus
DOI/URL:
Notes: --- - "Cited By (since 1996): 10" - "Export Date: 17 November 2011" - "CODEN: SPPKC" - "Source: Scopus"
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  1. Salvador Gonzalez Rodriguez
    203 Rodriguez