Reduced ceftazidime-avibactam susceptibility in KPC-producing Klebsiella pneumoniae from patients without ceftazidime-avibactam use history – A multicenter study in China Journal Article
| Authors: | Cui, X.; Shan, B.; Zhang, X.; Qu, F.; Jia, W.; Huang, B.; Yu, H.; Tang, Y. W.; Chen, L.; Du, H. |
| Article Title: | Reduced ceftazidime-avibactam susceptibility in KPC-producing Klebsiella pneumoniae from patients without ceftazidime-avibactam use history – A multicenter study in China |
| Abstract: | KPC-producing Klebsiella pneumoniae (KPC-KP) is the most widely spread carbapenem-resistant Enterobacteriaceae (CRE) in China. Avibactam is a novel non-β-lactam β-lactamase inhibitor which is highly active against KPC. Recently, ceftazidime-avibactam (CAZ-AVI) was approved for clinical treatment in China. Here we conducted a retrospective study to examine the antimicrobial susceptibility of CAZ-AVI prior to its usage in China, and evaluated the potential to develop resistance in KPC-KP. CAZ-AVI MICs were tested in 347 KPC-KP isolates collected from patients with no prior treatment with this combination from six medical centers in China. Almost all isolates (n = 346; 99.7%) were CAZ-AVI-susceptible, with only 12 (3.5%) which showed reduced susceptibility (MIC ≥ 4/4 μg/ml) or resistance. The 12 isolates belong to ST11 and half of them carry virulence genes. In comparison to susceptible isolates, these isolates demonstrated higher blaKPC–2 copy numbers and expressions, and demonstrated higher frequency of developing CAZ-AVI resistance. © Copyright © 2020 Cui, Shan, Zhang, Qu, Jia, Huang, Yu, Tang, Chen and Du. |
| Keywords: | adolescent; adult; child; clinical article; controlled study; aged; nonhuman; polymerase chain reaction; retrospective study; antibiotic resistance; infant; multicenter study; escherichia coli; newborn; ciprofloxacin; real time polymerase chain reaction; gentamicin; gene dosage; rna 16s; matrix assisted laser desorption ionization time of flight mass spectrometry; tetracycline; cotrimoxazole; levofloxacin; pulsed field gel electrophoresis; china; moxifloxacin; piperacillin plus tazobactam; bacterial gene; colistin; cefazolin; cefepime; sultamicillin; ceftazidime; minimum inhibitory concentration; amoxicillin plus clavulanic acid; meropenem; klebsiella pneumoniae; imipenem; amikacin; chloramphenicol; piperacillin; disk diffusion; rna polymerase beta subunit; sanger sequencing; human; article; antimicrobial resistance; rna sequencing; antimicrobial susceptibility; aztreonam; avibactam plus ceftazidime; porin; carbapenemase; ceftazidime-avibactam; mic50; multilocus sequence typing; cefotaxime; carbapenemase producing enterobacteriaceae; kpc; extended spectrum beta lactamase; ureidopenicillin; carbapenem resistant klebsiella pneumoniae; iuta; rmpa; rmpa2 |
| Journal Title: | Frontiers in Microbiology |
| Volume: | 11 |
| ISSN: | 1664-302X |
| Publisher: | Frontiers Media S.A. |
| Date Published: | 2020-06-23 |
| Start Page: | 1365 |
| Language: | English |
| DOI: | 10.3389/fmicb.2020.01365 |
| PROVIDER: | scopus |
| PMCID: | PMC7324628 |
| PUBMED: | 32655534 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 3 August 2020 -- Source: Scopus |
