Clinical relevance and functional significance of cell-free microRNA-1260b expression profiles in infiltrative myxofibrosarcoma Journal Article


Authors: Morita, T.; Fujiwara, T.; Yoshida, A.; Uotani, K.; Kiyono, M.; Yokoo, S.; Hasei, J.; Kunisada, T.; Ozaki, T.
Article Title: Clinical relevance and functional significance of cell-free microRNA-1260b expression profiles in infiltrative myxofibrosarcoma
Abstract: Infiltrative tumor growth into adjacent soft tissues is a major cause of the frequent recurrence and tumor-related death of myxofibrosarcoma (MFS), but no useful biomarkers reflecting tumor burden and infiltrative growth are available. While emerging evidence suggests a diagnostic and functional role of extracellular/circulating microRNA (miRNA) in various malignant diseases, their significance in MFS patients remains unknown. Global miRNA profiling identified four upregulated miRNAs in MFS patient sera and culture media of MFS cells. Among these, serum miR-1260b level was significantly upregulated in patient serum discriminating from healthy individuals and closely correlated with clinical status and tumor dynamics in MFS-bearing mice. In addition, high miR-1260b expression in serum was correlated with radiological tail-like patterns, characteristic of the infiltrative MFS. The extracellular miR-1260b was embedded in tumor-derived extracellular vesicles (EVs) and promoted cellular invasion of MFS through the downregulation of PCDH9 in the adjacent normal fibroblasts. Collectively, circulating miR-1260b expression may represent a novel diagnostic target for tumor monitoring of this highly aggressive sarcoma. Moreover, EV-miR-1260b could act as a transfer messenger to adjacent cells and mediate the infiltrative growth of MFS, providing new insights into the mechanism of infiltrative nature via crosstalk between tumor cells and their microenvironment. © 2020, The Author(s).
Journal Title: Scientific Reports
Volume: 10
ISSN: 2045-2322
Publisher: Nature Publishing Group  
Date Published: 2020-06-10
Start Page: 9414
Language: English
DOI: 10.1038/s41598-020-66120-8
PUBMED: 32523124
PROVIDER: scopus
PMCID: PMC7287053
DOI/URL:
Notes: Article -- Source: Scopus
Altmetric
Citation Impact
MSK Authors