Probing myeloid cell dynamics in ischaemic heart disease by nanotracer hot-spot imaging Journal Article
| Authors: | Senders, M. L.; Meerwaldt, A. E.; van Leent, M. M. T.; Sanchez-Gaytan, B. L.; van de Voort, J. C.; Toner, Y. C.; Maier, A.; Klein, E. D.; Sullivan, N. A. T.; Sofias, A. M.; Groenen, H.; Faries, C.; Oosterwijk, R. S.; van Leeuwen, E. M.; Fay, F.; Chepurko, E.; Reiner, T.; Duivenvoorden, R.; Zangi, L.; Dijkhuizen, R. M.; Hak, S.; Swirski, F. K.; Nahrendorf, M.; Pérez-Medina, C.; Teunissen, A. J. P.; Fayad, Z. A.; Calcagno, C.; Strijkers, G. J.; Mulder, W. J. M. |
| Article Title: | Probing myeloid cell dynamics in ischaemic heart disease by nanotracer hot-spot imaging |
| Abstract: | Ischaemic heart disease evokes a complex immune response. However, tools to track the systemic behaviour and dynamics of leukocytes non-invasively in vivo are lacking. Here, we present a multimodal hot-spot imaging approach using an innovative high-density lipoprotein-derived nanotracer with a perfluoro-crown ether payload (19F-HDL) to allow myeloid cell tracking by 19F magnetic resonance imaging. The 19F-HDL nanotracer can additionally be labelled with zirconium-89 and fluorophores to detect myeloid cells by in vivo positron emission tomography imaging and optical modalities, respectively. Using our nanotracer in atherosclerotic mice with myocardial infarction, we observed rapid myeloid cell egress from the spleen and bone marrow by in vivo 19F-HDL magnetic resonance imaging. Concurrently, using ex vivo techniques, we showed that circulating pro-inflammatory myeloid cells accumulated in atherosclerotic plaques and at the myocardial infarct site. Our multimodality imaging approach is a valuable addition to the immunology toolbox, enabling the study of complex myeloid cell behaviour dynamically. © 2020, The Author(s), under exclusive licence to Springer Nature Limited. |
| Keywords: | controlled study; nonhuman; flow cytometry; positron emission tomography; magnetic resonance imaging; animal cell; mouse; cytology; animal tissue; cell function; bone marrow; spleen; heart disease; molecular dynamics; in vivo study; cell specificity; immune response; heart infarction; atherosclerosis; radiation measurement; bone; cell migration; cellular distribution; bone marrow cell; tracer; mammals; cell interaction; atherosclerotic plaque; diseases; zirconium 89; ischemic heart disease; autoradiography; myocardial infarction; fluorescence imaging; cell selection; cell tracking; cells; cardiology; myeloid cells; gamma radiation; multimodal imaging; nanomaterial; high density lipoprotein; radiolabeling; female; priority journal; article; multi-modality imaging; crown ethers; myocardial infarcts; perfluoro compound; apolipoprotein e knockout mouse; fluorine magnetic resonance imaging |
| Journal Title: | Nature Nanotechnology |
| Volume: | 15 |
| Issue: | 5 |
| ISSN: | 1748-3387 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2020-05-01 |
| Start Page: | 398 |
| End Page: | 405 |
| Language: | English |
| DOI: | 10.1038/s41565-020-0642-4 |
| PUBMED: | 32313216 |
| PROVIDER: | scopus |
| PMCID: | PMC7416336 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 June 2020 -- Source: Scopus |
