Long-term in vivo biocompatibility of single-walled carbon nanotubes Journal Article
| Authors: | Galassi, T. V.; Antman-Passig, M.; Yaari, Z.; Jessurun, J.; Schwartz, R. E.; Heller, D. A. |
| Article Title: | Long-term in vivo biocompatibility of single-walled carbon nanotubes |
| Abstract: | Over the past two decades, measurements of carbon nanotube toxicity and biodistribution have yielded a wide range of results. Properties such as nanotube type (single-walled vs. multi-walled), purity, length, aggregation state, and functionalization, as well as route of administration, greatly affect both the biocompatibility and biodistribution of carbon nanotubes. These differences suggest that generalizable conclusions may be elusive and that studies must be material- and application-specific. Here, we assess the short- and long-term biodistribution and biocompatibility of a single-chirality DNA-encapsulated single-walled carbon nanotube complex upon intravenous administration that was previously shown to function as an in-vivo reporter of endolysosomal lipid accumulation. Regarding biodistribution and fate, we found bulk specificity to the liver and >90% signal attenuation by 14 days in mice. Using near-infrared hyperspectral microscopy to measure single nanotubes, we found low-level, long-term persistence in organs such as the heart, liver, lung, kidney, and spleen. Measurements of histology, animal weight, complete blood count; biomarkers of organ function all suggest short- and long-term biocompatibility. This work suggests that carbon nanotubes can be used as preclinical research tools in-vivo without affecting acute or long-term health. © 2020 Galassi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| Keywords: | controlled study; microscopy; nonhuman; biological marker; mouse; animal tissue; spleen; animal experiment; body weight; in vivo study; histology; liver; kidney; lung; biocompatibility; blood cell count; single stranded dna; heart; encapsulation; single walled nanotube; lipid storage; male; article; organ distribution |
| Journal Title: | PLoS ONE |
| Volume: | 15 |
| Issue: | 5 |
| ISSN: | 1932-6203 |
| Publisher: | Public Library of Science |
| Date Published: | 2020-05-06 |
| Start Page: | e0226791 |
| Language: | English |
| DOI: | 10.1371/journal.pone.0226791 |
| PUBMED: | 32374764 |
| PROVIDER: | scopus |
| PMCID: | PMC7202660 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 June 2020 -- Source: Scopus |
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