A near-infrared probe for non-invasively monitoring cerebrospinal fluid flow by (18)F-positron emitting tomography and fluorescence Journal Article
| Authors: | Guo, H.; Kommidi, H.; Lekaye, C. C.; Koutcher, J.; Judenhofer, M. S.; Cherry, S. R.; Wu, A. P.; Akin, O.; Souweidane, M. M.; Aras, O.; Zhu, Z.; Ting, R. |
| Article Title: | A near-infrared probe for non-invasively monitoring cerebrospinal fluid flow by (18)F-positron emitting tomography and fluorescence |
| Abstract: | Purpose: Knowing the precise flow of cerebrospinal fluid (CSF) is important in the management of multiple neurological diseases. Technology for non-invasively quantifying CSF flow would allow for precise localization of injury and assist in evaluating the viability of certain devices placed in the central nervous system (CNS). Methods: We describe a near-infrared fluorescent dye for accurately monitoring CSF flow by positron emission tomography (PET) and fluorescence. IR-783, a commercially available near-infrared dye, was chemically modified and radiolabeled with fluorine-18 to give [18F]-IR783-AMBF3. [18F]-IR783-AMBF3 was intrathecally injected into the rat models with normal and aberrant CSF flow and evaluated by the fluorescence and PET/MRI or PET/CT imaging modes. Results: IR783-AMBF3 was clearly distributed in CSF-containing volumes by PET and fluorescence. We compared IR783-AMBF3 (fluorescent at 778/793 nm, ex/em) to a shorter-wavelength, fluorescein equivalent (fluorescent at 495/511 nm, ex/em). IR783-AMBF3 was superior for its ability to image through blood (hemorrhage) and for imaging CSF-flow, through-skin, in subdural-run lumboperitoneal shunts. IR783-AMBF3 was safe under the tested dosage both in vitro and in vivo. Conclusion: The superior imaging properties of IR783-AMBF3 could lead to enhanced accuracy in the treatment of patients and would assist surgeons in non-invasively diagnosing diseases of the CNS. © 2020, The Author(s). |
| Keywords: | positron emission tomography; fluorescence; csf flow; cns disease; non-invasively |
| Journal Title: | EJNMMI Research |
| Volume: | 10 |
| ISSN: | 2191-219X |
| Publisher: | Springer |
| Date Published: | 2020-01-01 |
| Start Page: | 37 |
| Language: | English |
| DOI: | 10.1186/s13550-020-0609-3 |
| PROVIDER: | scopus |
| PMCID: | PMC7163004 |
| PUBMED: | 32301036 |
| DOI/URL: | |
| Notes: | Article -- Source: Scopus |
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