Initial evaluation of a new epithelial antigen (T16) for bivariate flow cytometry of bladder irrigation specimens Journal Article

  


Authors: Bretton, P. R.; Myc, A.; Cordon‐Cardo, C.; DeAngelis, P.; Fair, W. R.; Melamed, M. R.
Article Title: Initial evaluation of a new epithelial antigen (T16) for bivariate flow cytometry of bladder irrigation specimens
Abstract: Bivariate flow cytometry (FCM) was used to study immunofluorescent T16 mouse monoclonal antibody (Mab) binding simultaneously with propidium iodide DNA measurements in bladder irrigation specimens from 30 patients with a history of bladder cancer. Aliquots of the same samples were stained with acridine orange (AO) and examined by conventional FCM. T16 Mab is believed to be specific for epithelial cells in this type of specimen and stained from 13% of the cells in a patient with cystitis to 95% of the cells in a patient with an atypical papilloma. In combination with DNA measurements, this antibody increased the sensitivity of FCM in patients with severe cystitis and relatively small numbers of tumor cells, but the diagnostic specificity may be decreased and the criteria established for interpreting univariate flow cytometry may have to be re‐evaluated and modified. Copyright © 1989 Wiley‐Liss, Inc.
Keywords: clinical article; flow cytometry; cytology; bladder cancer; monoclonal antibodies; monoclonal antibody; antibodies, monoclonal; dna, neoplasm; carcinoma; bladder neoplasms; cytochemistry; epithelial membrane antigen; human; propidium; support, u.s. gov't, p.h.s.; diagnostic specificity
Journal Title: Cytometry
Volume: 10
Issue: 3
ISSN: 0196-4763
Publisher: Wiley Liss  
Date Published: 1989-05-01
Start Page: 339
End Page: 344
Language: English
DOI: 10.1002/cyto.990100314
PUBMED: 2714117
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0024573837&doi=10.1002%2fcyto.990100314&partnerID=40&md5=08b1f942db0670a8f6eb9ac66d94603f
Notes: Article -- Export Date: 14 April 2020 -- Source: Scopus
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MSK Authors
  1. Carlos Cordon-Cardo
    612 Cordon-Cardo
  2. William R Fair
    342 Fair
  3. Myron Melamed
    148 Melamed
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