Decreased content of the 35kDa cytoskeletal protein p35 in friend erythroleukemia cells exposed to dimethyl sulfoxide and retinoic acid is associated with entrance into a quiescent substrate Journal Article

  

Authors:	Higgins, P. J.; Lipkin, M.; Bueti, C.; Traganos, F.
Article Title:	Decreased content of the 35kDa cytoskeletal protein p35 in friend erythroleukemia cells exposed to dimethyl sulfoxide and retinoic acid is associated with entrance into a quiescent substrate
Abstract:	1. 1. The effect of all-traw-retinoic acid (RA) on cell cycle kinetics, RNA content, and expression of the 35 kDa cytoskeletal protein p35 in exponentially-growing Friend erythroleukemia (FL) cells was compared with the prototypic differentiation-inducer dimethylsulfoxide (DMSO). 2. 2. Two g1 phase populations of RA-treated FL cells were identified: one with an intermediate RNA content (T-cells) similar to G1 cells in near-plateau-phase control cultures and the other with a very low RNA content (Q-cells) similar to DMSO-differentiated cells; although quiescent, RA-treated cells remained undifferentiated as evidenced by the absence of late-stage markers of erythroid maturation. 3. 3. Decreases in the cellular content of p35 occurred in both DMSO- and RA-treated FL cells, correlating with the onset of accumulation of cells into G1, and stabilized by 48 hr after initial exposure to either inducer. 4. 4. Down-regulation in the cellular p35 content, thus, appears to be linked to entrance of FL cells into a quiescent substrate and independent of the subsequent capacity for erythroid differentiation. © 1989.
Keywords:	animal; mice; cell cycle; cell line; dimethyl sulfoxide; kinetics; cell culture; dna, neoplasm; molecular weight; retinoic acid; cytoskeleton protein; staining and labeling; cytoskeletal proteins; interphase; tretinoin; leukemia, erythroblastic, acute; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; support, u.s. gov't, non-p.h.s.; erythroleukemia cell; friend murine leukemia virus; protein p 35
Journal Title:	International Journal of Biochemistry
Volume:	21
Issue:	6
ISSN:	0020-711X
Publisher:	Elsevier Inc.
Date Published:	1989-01-01
Start Page:	619
End Page:	626
Language:	English
DOI:	10.1016/0020-711x(89)90380-7
PUBMED:	2477289
PROVIDER:	scopus
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0024362170&doi=10.1016%2f0020-711X%2889%2990380-7&partnerID=40&md5=75c473067eccbef5b25210c93cdea302
Notes:	Article -- Export Date: 14 April 2020 -- Source: Scopus

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