Membrane-anchored and soluble forms of betaglycan, a polymorphic proteoglycan that binds transforming growth factor-β Journal Article
| Authors: | Andres, J. L.; Stanley, K.; Cheifetz, S.; Massagué, J. |
| Article Title: | Membrane-anchored and soluble forms of betaglycan, a polymorphic proteoglycan that binds transforming growth factor-β |
| Abstract: | Transforming growth factors β1 and β2 bind with high affinity to the core protein of a 250-350-kD cell surface proteoglycan. This proteoglycan (formerly referred to as the type III TGF-β receptor) coexists in many cells with the receptor implicated in TGF-β signal transduction (type I TGF-β receptor), but its function is not known. We report here that solution TGF-β-binding proteoglycans are released by several cell types into the culture media, and can be found in serum and extracellular matrices. As has been shown for the membrane-bound form, the soluble proteoglycans have a heterogeneous core protein of 100-120 kD that carries chondroitin sulfate and/or heparan sulfate glycosaminoglycan chains and a small amount of N-linked carbohydrate. The membrane-bound form of this proteoglycan is hydrophobic and associates with liposomes, whereas the soluble forms lack a membrane anchor and do not associate with liposomes. Differences in the electrophoretic migration of the soluble and membrane forms of this proteoglycan suggest additional structural differences in their core proteins and glycosaminoglycan chains. These soluble and membrane-bound proteoglycans, for which we propose the name 'betaglycans', might play distinct roles in pericellular retention, delivery, or clearance of activated TGF-β. |
| Keywords: | nonhuman; animal cell; animal; cells, cultured; transforming growth factor beta; cell line; protein binding; animalia; kinetics; cell culture; cell membrane; transforming growth factors; beta glucan; molecular weight; cytosol; liposomes; chromatography, affinity; membrane receptor; proteoglycans; proteoglycan; chromatography, ion exchange; human; priority journal; article; support, u.s. gov't, p.h.s. |
| Journal Title: | Journal of Cell Biology |
| Volume: | 109 |
| Issue: | 6 |
| ISSN: | 0021-9525 |
| Publisher: | Rockefeller University Press |
| Date Published: | 1989-12-01 |
| Start Page: | 3137 |
| End Page: | 3145 |
| Language: | English |
| DOI: | 10.1083/jcb.109.6.3137 |
| PUBMED: | 2592419 |
| PROVIDER: | scopus |
| PMCID: | PMC2115961 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 14 April 2020 -- Source: Scopus |
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