| Abstract: |
Weanalyzed the effects of 3'-azido-3'-deoxythymidine(AZT) and a and y interferon (lFN-a and IFN-y) on transformation of human umbilical cord lymphocytes (HUCLs) by EpsteinBarr virus (EBV). B lymphocytic outgrowth from HUCLs infected with EBV was monitored by focus formation; the presence of EBV genomes in transformed foci was confirmed by in situ cytohybridization and EBV nuclear antigen. The 50% inhibitory doses for preventing focus formation were 0.14 J.lM for AZT, 10 units (U)/mL for IFN-a, and 1 U/mL for IFN-y. To determine whether inhibition resulted from direct cytotoxic effects, weassessed the viability of non transformed HUCLs exposed to the agents for 30 d. The nontoxic doses were 1 J.lM for AZT, 30 U/mL for IFN-a, and 3 U/mL for IFN-y. Combining IFN-a or IFN-y with AZT or IFN-a with IFN-y at nontoxic doses showed synergistic decreases in numbers of transformed foci. Transformation of HUCLs by EBV was sensitive to, but not abolished by, these agents at doses below those required for cell killing. © 1989 by The University of Chicago. All Rights Reserved. |
| Keywords: |
dose response; drug potentiation; alpha interferon; cell survival; cells, cultured; cell growth; cytotoxicity; dose-response relationship, drug; b lymphocyte; b-lymphocytes; drug synergism; gamma interferon; drug combination; cell transformation; umbilical cord blood; computer program; epstein barr virus; cell transformation, viral; herpesvirus 4, human; zidovudine; computer analysis; virus inhibition; interferon type i; interferon type ii; human; priority journal; support, u.s. gov't, p.h.s.
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