Synergistic antiproliferative effect of interferon alpha and azidothymidine in chronic myelogenous leukemia Journal Article
| Authors: | Estrov, Z.; Talpaz, M.; Chou, T. C.; Kurzrock, R.; Blake, M.; Gutterman, J. U. |
| Article Title: | Synergistic antiproliferative effect of interferon alpha and azidothymidine in chronic myelogenous leukemia |
| Abstract: | The possible synergistic interaction between azidothymidine (AZT) and interferon alpha (rIFN-alpha-2a) in the treatment of chronic myelogenous leukemia (CML) was studied in vitro using marrow or peripheral blood hematopoietic progenitors from 10 patients with CML in the mixed (CFU-GEMM) colony culture assay. Used singly, either agent inhibited erythroid (BFU-E) and granulocyte-macrophage (CFU-GM) CML hematopoietic progenitor proliferation in a dose-dependent fashion, with the inhibitory effect being more pronounced on BFU-E than on CFU-GM colony-forming cells. The combination of both drugs in therapeutic concentrations exerted a significant synergistic inhibition on CML stem cells as assessed by the median-effect principle and isobologram equation analysis. A suboptimal dose of AZT (0.5-mu-mol/1) synergistically augmented the effect of rIFN-alpha-2a whereas an inactive dose of 10 U/ml rIFN-alpha-2a similarly enhanced the CML stem cell growth inhibition exerted by AZT. Our data indicate that AZT may augment the already established therapeutic benefits of IFN-alpha in CML. |
| Keywords: | virus; placebo-controlled trial; chronic; myeloid-leukemia; hematopoietic progenitor cells; invitro; human-bone-marrow; aids-related complex; a interferon; platelet dna-polymerase; recombinant leukocyte; culture reveals |
| Journal Title: | Leukemia |
| Volume: | 5 |
| Issue: | 2 |
| ISSN: | 0887-6924 |
| Publisher: | Nature Publishing Group |
| Date Published: | 1991-02-01 |
| Start Page: | 101 |
| End Page: | 107 |
| Language: | English |
| ACCESSION: | WOS:A1991FH64700002 |
| PROVIDER: | wos |
| PUBMED: | 2020193 |
| Notes: | Source: Wos |
