Single-cell functional proteomics microchip for profiling circulating hematopoietic stem/progenitor cells and early detection of myelofibrosis Conference Paper
| Authors: | Chen, J.; Kim, D.; Chen, Z.; Kleppe, M.; Levine, R.; Fan, R. |
| Title: | Single-cell functional proteomics microchip for profiling circulating hematopoietic stem/progenitor cells and early detection of myelofibrosis |
| Conference Title: | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 |
| Abstract: | We developed a single-cell protein secretion profiling technology to measure the function of rare circulating hematopoetic stem/progenitor cells (HPSCs) in patients with myeloproliferative neoplasm (MPN). It is a microchip platform the allows for highly multiplexed (up to 42) cytokine production measurement in single cells. It was applied to a prospective cohort of ∼60 patients for detecting abnormal cytokine-secreting hematopoietic cells in blood. The results revealed the potential to discover new biomarkers based on single-cell protein signatures for early diagnosis of disease progression. Three new biomarkers we identified and demonstrated the ability to detect fibrotic progression of patients from non-fibrotic stages (P value < 0.01) as well as to stratify patients with closely related conditions for precision medicine. Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved. |
| Keywords: | proteins; biomarkers; cytology; cytokine; cytokines; disease progression; diagnosis; cytokine production; molecular biology; cells; single-cell analysis; hematopoietic stem/progenitor cells; microprocessor chips; singlecell analysis; myeloid proliferative disease; profiling technology; proliferative disease; single cell proteins |
| Journal Title | Proceedings of the 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018) |
| Conference Dates: | 2018 Nov 11-15 |
| Conference Location: | Kaohsiung, Taiwan |
| ISBN: | 9781510897571 |
| Publisher: | Chemical and Biological Microsystems Society |
| Date Published: | 2018-01-01 |
| Start Page: | 1924 |
| End Page: | 1926 |
| Language: | English |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Conference Paper -- Export Date: 1 April 2020 -- Source: Scopus |
