Genetic modification of human hematopoietic cells: Preclinical optimization of oncoretroviral-mediated gene transfer for clinical trials Journal Article
| Authors: | Budak-Alpdogan, T.; Rivière, I. |
| Article Title: | Genetic modification of human hematopoietic cells: Preclinical optimization of oncoretroviral-mediated gene transfer for clinical trials |
| Abstract: | This chapter provides information about the oncoretroviral transduction of human hematopoietic stem/progenitor cells under clinically applicable conditions. We describe in detail a short –60 h transduction protocol which consistently yields transduction efficiencies in the range of 30–50% with five different oncoretroviral vectors. We discuss a number of parameters that affect transduction efficiency, including the oncoretroviral vector characteristics, the vector stock collection, the source of CD34+ cells and transduction conditions. © Humana Press, a part of Springer Science + Business Media, LLC 2009. |
| Keywords: | human cell; genetics; clinical trial; polymerase chain reaction; metabolism; cell viability; cells, cultured; cd34 antigen; culture medium; cell line; gene transfer; gene vector; genetic transduction; genetic vectors; transduction, genetic; immunology; dna modification; cell culture; retrovirus vector; hematopoietic stem cells; hematopoietic stem cell; cell stimulation; antigens, cd34; cd34 selection; inoculation; retrovirus; retroviridae; gene transfer techniques; transduction; tissue culture; process optimization; culture media, serum-free; retroviral gene transfer; humans; human; article; cd34+ cells; polycation; oncoretroviral vector; preclinical optimization; retronectin; tissue culture bags; vector production |
| Journal Title: | Methods in Molecular Biology |
| Volume: | 506 |
| ISSN: | 1064-3745 |
| Publisher: | Humana Press Inc |
| Date Published: | 2009-01-01 |
| Start Page: | 33 |
| End Page: | 58 |
| Language: | English |
| DOI: | 10.1007/978-1-59745-409-4_4 |
| PUBMED: | 19110618 |
| PROVIDER: | scopus |
| PMCID: | PMC4360985 |
| DOI/URL: | |
| Notes: | Book Chapter 4 in "Genetic Modification of Hematopoietic Stem Cells: Methods and Protocols" (ISBN: 978-1-58829-980-2) -- Export Date: 6 March 2020 -- Source: Scopus |
