Human monoclonal antibody with dual GM2/GD2 specificity derived from an immunized melanoma patient Journal Article
| Authors: | Yamaguchi, H.; Furukawa, K.; Fortunato, S. R.; Livingston, P. O.; Lloyd, K. O.; Oettgen, H. F.; Old, L. J. |
| Article Title: | Human monoclonal antibody with dual GM2/GD2 specificity derived from an immunized melanoma patient |
| Abstract: | GM2 ganglioside is a common cell surface constituent of human melanoma and other tumors of neuroectodermal origin, and vaccination with GM2 ganglioside results in high levels of anti-GM2 antibodies in patients with melanoma. Lymphocytes from a GM2-vaccinated patient (VS) were transformed by Epstein-Barr virus and tested for production of antibodies with reactivity for GM2-positive tumor cells. A high percentage of antibody-producing B cells was detected, but antibody reactivity was generally lost during culture expansion. Two cultures, however, remained stable for antibody productivity and one was used to develop a stable hybrid line with mouse myeloma. The monoclonal antibody (designated 3-207) derived from patient VS has dual specificity for GM2 and GD2, despite the fact that only GM2 antibody could be detected in the patient's serum. Monoclonal antibody 3-207 shows high-titered reactivity with a range of melanoma, astrocytoma, neuroblastoma, and leukemia cell lines, cells with prominent cell surface expression of GM2 and GD2. The cell surface reactivity of monoclonal antibody 3-207 was not abolished by treatment of target cells with neuraminidase, as the enzyme converted GD2 to GM2, which was still detected by monoclonal antibody 3-207. |
| Keywords: | case report; animal; mice; bcg vaccine; melanoma; cell line; tumor cells, cultured; monoclonal antibody; antibodies, monoclonal; antibody specificity; ganglioside gm3; neuraminidase; ganglioside gd3; antibody production; vaccines; cell transformation, viral; herpesvirus 4, human; cell fusion; immunization; gangliosides; tissue culture; human herpesvirus 4; cancer immunology; g(m2) ganglioside; chromatography, thin layer; human; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; epstein-barr virus transformation |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 87 |
| Issue: | 9 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1990-05-01 |
| Start Page: | 3333 |
| End Page: | 3337 |
| Language: | English |
| DOI: | 10.1073/pnas.87.9.3333 |
| PUBMED: | 2159145 |
| PROVIDER: | scopus |
| PMCID: | PMC53894 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 27 January 2020 -- Source: Scopus |
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