Vaccines containing purified GM2 ganglioside elicit GM2 antibodies in melanoma patients Journal Article

   


Authors: Livingston, P. O.; Natoli, E. J.; Calves, M. J.; Stockert, E.; Oettgen, H. F.; Old, L. J.
Article Title: Vaccines containing purified GM2 ganglioside elicit GM2 antibodies in melanoma patients
Abstract: GM2, GD2, and GD3 gangliosides are expressed on the surface of human melanoma cells and represent potential targets for immunological control of melanoma growth by monoclonal antibodies and active immunization. The immunogenicity of GM2 was investigated by analyzing the humoral immune response of melanoma patients to vaccination with cell lines selected for high GM2 expression and with vaccines containing purified GM2. The whole-cell vaccine and vaccines containing purified GM2 and bacillus Calmette-Guerin (BCG) elicited GM2 antibody in a high proportion of patients, particularly in GM2/BCG-vaccinated patients pretreated with cyclophosphamide and given a GM2/BCG booster immunization. Vaccines containing purified GM2 and Salmonella minnesota R595 as the adjuvant were also effective, but only in patients pretreated with cyclophosphamide. GM2 antibodies in vaccinated patients were of the IgM class and were cytotoxic for GM2-positive targets in the presence of human complement.
Keywords: human cell; animals; mice; melanoma; biological model; cell line; monoclonal antibody; membrane antigen; immunotherapy; cancer vaccine; melanoma cell; melanoma, experimental; ganglioside gm2; antibody formation; vaccines; human experiment; immunoglobulin m; gangliosides; salmonella; active immunization; g(m2) ganglioside; mycobacterium bovis bcg; humans; human; priority journal; blood and hemopoietic system; salmonella enterica subsp. enterica serovar minnesota
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 84
Issue: 9
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 1987-05-01
Start Page: 2911
End Page: 2915
Language: English
DOI: 10.1073/pnas.84.9.2911
PUBMED: 3472242
PROVIDER: scopus
PMCID: PMC304770
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0000221296&doi=10.1073%2fpnas.84.9.2911&partnerID=40&md5=1bd55ce5897b24bf12793454f5e6df60
Notes: Article -- Export Date: 5 February 2021 -- Source: Scopus
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MSK Authors
  1. Philip O Livingston
    228 Livingston
  2. Herbert F Oettgen
    130 Oettgen
  3. Lloyd J Old
    593 Old
  4. Elisabeth Stockert
    87 Stockert
  5. Michele Jones Calves
    15 Jones Calves
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