Interleukin 2 gene transfer into tumor cells abrogates tumorigenicity and induces protective immunity Journal Article

   


Authors: Gansbacher, B.; Zier, K.; Daniels, B.; Cronin, K.; Bannerji, R.; Gilboa, E.
Article Title: Interleukin 2 gene transfer into tumor cells abrogates tumorigenicity and induces protective immunity
Abstract: To study the effects of localized secretion of cytokines on tumor progression, the gene for human interleukin 2 (IL-2) was introduced via retroviral vectors into CMS-5 cells, a weakly immunogenic mouse fibrosarcoma cell line of BALB/c origin. Secretion of low levels of IL-2 from the tumor cells abrogated their tumorigenicity and induced a long-lasting protective immune response against a challenge with a tumorigenic dose of parental CMS-5 cells. Co-injection of IL-2-producing CMS-5 cells with unmodified tumor cells inhibited tumor formation even when highly tumorigenic doses of CMS-5 cells were used. Cytolytic activity in mice injected with parental CMS-5 cells was transient and was greatly diminished 3 wk after injection, as commonly observed in tumor-bearing animals. However, in mice injected with IL-2-producing cells, tumor-specific cytolytic activity persisted at high levels for the duration of the observation period (at least 75 d). High levels of tumor-specific cytolytic activity could also be detected in parental CMS-5 tumor-bearing animals 18 d after inoculation with tumor cells, if IL-2-producing CMS-5 cells but not unmodified parental tumor cells were used as targets. These studies highlight the potential advantages of localized secretion of cytokines mediated via gene transfer to induce potent anti-tumor immune responses. © 1990, Rockefeller University Press., All rights reserved.
Keywords: nonhuman; animal cell; mouse; animal; mice; interleukin 2; animal experiment; tumor cells, cultured; transfection; mice, inbred balb c; gene transfer; cell culture; t-lymphocytes, cytotoxic; carcinogenicity; neoplasms, experimental; tumor immunity; cytotoxicity, immunologic; interleukin-2; human; priority journal; article; support, non-u.s. gov't
Journal Title: Journal of Experimental Medicine
Volume: 172
Issue: 4
ISSN: 0022-1007
Publisher: Rockefeller University Press  
Date Published: 1990-10-01
Start Page: 1217
End Page: 1224
Language: English
DOI: 10.1084/jem.172.4.1217
PUBMED: 2212951
PROVIDER: scopus
PMCID: PMC2188618
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025000864&doi=10.1084%2fjem.172.4.1217&partnerID=40&md5=9f99bd3193743fd9882689b9effa34d4
Notes: Article -- Export Date: 27 January 2020 -- Source: Scopus
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MSK Authors
  1. Bernd Gansbacher
    62 Gansbacher
  2. Eli Gilboa
    46 Gilboa
  3. Kathryn C. Cronin
    14 Cronin
  4. Rajat Bannerji
    11 Bannerji
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