A novel autoregulatory cytokine is required for the regulation of autoaggressive responses Journal Article

  


Authors: Rosenkrantz, K.; Bhimani, K.; Welte, K.; Buck, J.; DiSanto, J.; Levi, E.; DiMartino, J.; Hammerling, U.; Dupont, B.; Flomenberg, N.
Article Title: A novel autoregulatory cytokine is required for the regulation of autoaggressive responses
Abstract: Limiting dilution studies indicate that cells with the potential to lyse autologous target cells exist in the peripheral blood of all normal individuals. In contrast to allocytotoxic cells, autocytolytic cells are down-regulated by a second less frequent cell population. When recombinant interleukin 2 is substituted for crude lymphocyte conditioned medium in these limiting dilution experiments, autocytotoxicity develops normally. Under these conditions, however, the autocytotoxic response is not down-regulated. Mixing crude lymphocyte-conditioned medium together with recombinant interleukin 2 restores the regulation of autocytotoxicity normally seen at high responder cell dose. These findings indicate that a second soluble factor present in the conditioned medium is necessary either for the activation, growth, or differentiation of the regulatory cell population or alternatively, to render the cytotoxic population responsive to the activity of regulatory cells. Gel filtration studies indicate that the molecular weight of this factor is between 60 and 80 kd. This factor appears to be distinct from known immunologically active cytokines. It is conceivable that deficiencies of this cytokine may be relevant to the pathogenesis of autoimmune diseases or graft-versus-host reactions. © 1990.
Keywords: human cell; t-lymphocytes; cells, cultured; cytotoxicity; immunoregulation; cytokine; cytokines; cell culture; recombinant proteins; graft versus host reaction; autoimmunity; cytotoxicity, immunologic; molecular weight; interleukin-2; chromatography, gel; biological factors; tetradecanoylphorbol acetate; human; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.
Journal Title: Human Immunology
Volume: 27
Issue: 3
ISSN: 0198-8859
Publisher: Elsevier Inc.  
Date Published: 1990-03-01
Start Page: 254
End Page: 264
Language: English
DOI: 10.1016/0198-8859(90)90055-t
PUBMED: 2187837
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025174689&doi=10.1016%2f0198-8859%2890%2990055-T&partnerID=40&md5=93b3bc6435530e26723605e9f05913ca
Notes: Source: Scopus
Altmetric
What is Altmetric?
Citation Impact
What is Dimensions Citation Badge?
BMJ Impact Analytics
MSK Authors
  1. Ulrich Hammerling
    79 Hammerling
  2. Bo Dupont
    264 Dupont
  3. Ester Levi
    17 Levi
  4. Neal Flomenberg
    56 Flomenberg
  5. Karl Welte
    77 Welte
  6. James Di Santo
    16 Di Santo
  7. Jochen Buck
    13 Buck
Related MSK Work