Up-regulation by feline interleukin-4 and down-regulation by feline interferon-gamma of major histocompatibility complex class II on cat B-lymphocytes Journal Article
| Authors: | Gomes-Keller, M. A.; Nunez, R.; Schroff, M.; Oswald, D.; Willett, B. J.; Lutz, H. |
| Article Title: | Up-regulation by feline interleukin-4 and down-regulation by feline interferon-gamma of major histocompatibility complex class II on cat B-lymphocytes |
| Abstract: | Interleukin-4 (IL-4) exhibits numerous biological and immunoregulatory functions on B- and T-lymphocytes, monocytes, and dendritic cells in both mice and humans. In the present study, we show that IL-4 also has a regulatory function in the cat species. Cells transfected with IL-4 DNA expressed a biologically active protein as demonstrated by the up-regulation of MHC class II molecules on B-lymphocytes (CD21+) in a flow cytometric assay. Increased levels of MHC class II expression on CD21+ cells were seen in 11 out of 12 cats (p<0.05). In addition, 12 out of 12 cats showed up-regulation of MHC class II on CD21- cells, mainly consisting of T-lymphocytes (p<0.05). In contrast, concanavalin A (ConA)-induced culture supernatant from peripheral blood mononuclear cells (PBMCs) containing high levels of interferon-gamma (IFN-γ) transcripts induced down-regulation of MHC class II molecules on CD21+ cells of all samples (p<0.05). Variable results were observed for CD21- cells incubated with ConA-conditioned medium (p=0.71). The nature of the cytokine(s) responsible for these effects remains to be determined. However, the fact that down-regulation of MHC class II molecules on B cells occurred in all cats tested suggests that IFN-γ may be involved. These data provide further insight into the mechanism by which MHC class II expression is regulated in feline lymphocytes, and suggest that the Th1/Th2 paradigm is also present in the cat. © 2002 Elsevier Science B.V. All rights reserved. |
| Keywords: | controlled study; protein expression; genetics; nonhuman; flow cytometry; polymerase chain reaction; t lymphocyte; animal; metabolism; animals; cells, cultured; interleukin 4; culture medium; down-regulation; genetic transcription; transcription, genetic; transfection; b lymphocyte; b-lymphocytes; blood; th2 cell; biosynthesis; immunology; genetic transfection; mononuclear cell; gamma interferon; cell culture; cat; cats; nucleotide sequence; hla antigen class 2; major histocompatibility antigen class 2; down regulation; upregulation; interferon-gamma; th1 cell; up-regulation; histocompatibility antigens class ii; major histocompatibility complex; sampling; expression; interleukin-4; culture media, conditioned; complement component c3d receptor; receptors, complement 3d; felidae; interferon type ii; concanavalin a; felis catus; male; female; article; mhc class ii |
| Journal Title: | Veterinary Immunology and Immunopathology |
| Volume: | 88 |
| Issue: | 3-4 |
| ISSN: | 0165-2427 |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 2002-09-25 |
| Start Page: | 197 |
| End Page: | 208 |
| Language: | English |
| DOI: | 10.1016/s0165-2427(02)00171-x |
| PUBMED: | 12127417 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 14 November 2014 -- Source: Scopus |
