Direct estimation of serological H-Y antigen by flow cytometry Journal Article
| Authors: | Kent, M.; Wachtel, S.; Thaler, H. T. |
| Article Title: | Direct estimation of serological H-Y antigen by flow cytometry |
| Abstract: | This study was undertaken to evaluate the efficiency of flow cytometry in the detection of the serological H-Y antigen, and to survey expression of HY in the normal human population. Peripheral blood leukocytes (granulocytes) were reacted with monoclonal H-Y antibody, gw-16, and with FITC-conjugated goat anti-mouse IgG, and then were scored for fluorescence in the flow cytometer. Assigned H-Y phenotype was correlated accurately with sex phenotype in 33 out of 38 women and 62 out of 65 men. Data were evaluated by exploratory data analysis (EDA). © 1990 Springer-Verlag. |
| Keywords: | human cell; flow cytometry; methodology; reproducibility of results; phenotype; sex determination (analysis); fluorescent antibody technique; antibodies, monoclonal; predictive value of tests; sex difference; normal human; granulocytes; normal value; human; male; female; priority journal; article; support, u.s. gov't, p.h.s.; hy antigen; h-y antigen; capra hircus |
| Journal Title: | Human Genetics |
| Volume: | 85 |
| Issue: | 1 |
| ISSN: | 0340-6717 |
| Publisher: | Springer |
| Date Published: | 1990-06-01 |
| Start Page: | 127 |
| End Page: | 129 |
| Language: | English |
| DOI: | 10.1007/bf00276339 |
| PUBMED: | 2192980 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Source: Scopus |
