Cytogenetic analysis of human solid tumors by in situ hybridization with a set of 12 chromosome-specific DNA probes Journal Article

  


Authors: van Dekken, H.; Pizzolo, J. G.; Reuter, V. E.; Melamed, M. R.
Article Title: Cytogenetic analysis of human solid tumors by in situ hybridization with a set of 12 chromosome-specific DNA probes
Abstract: We have applied fluorescent in situ hybridization (FISH) to assess the presence of numerical chromosome aberrations in fresh specimens of human solid tumors of varying histology. For this purpose, a set of 12 biotinylated chromosome-specific. repetitive alpha-satellite DNA probes (for chromosomes 1.6.7. 9, 10. II. 15. 16. 17. 18. X. and Y) were hybridized directly to isolated interphase nuclei. Utilizing this approach, we found numerical chromosome changes in all tumors. FISH ploidy profiles were in accordance with flow cytometric DNA histograms of these tumor cells. © 1990 S. Karger AG, Basel.
Keywords: human tissue; case report; solid tumor; pancreatic neoplasms; neoplasms; fluorescence; sarcoma; prostatic neoplasms; in situ hybridization; chromosome aberrations; nucleic acid hybridization; chromosome analysis; chromosomes, human; karyotyping; repetitive sequences, nucleic acid; dysgerminoma; dna probes; human; male; priority journal; article; dna probe; dna, satellite
Journal Title: Cytogenetics and Cell Genetics
Volume: 54
Issue: 3-4
ISSN: 0301-0171
Publisher: S. Karger AG  
Date Published: 1990-01-01
Start Page: 103
End Page: 107
Language: English
DOI: 10.1159/000132971
PUBMED: 2265552
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025689326&doi=10.1159%2f000132971&partnerID=40&md5=a983b7618b339bb719ff7769426a253e
Notes: Source: Scopus
Altmetric
What is Altmetric?
Citation Impact
What is Dimensions Citation Badge?
BMJ Impact Analytics
MSK Authors
  1. Victor Reuter
    1228 Reuter
  2. Myron Melamed
    148 Melamed
Related MSK Work