Role of transforming growth factor ß1 in induction of colon carcinoma differentiation by hexamethylene bisacetamide Journal Article
| Authors: | Schroy, P.; Rifkin, J.; Coffey, R. J.; Winawer, S.; Friedman, E. |
| Article Title: | Role of transforming growth factor ß1 in induction of colon carcinoma differentiation by hexamethylene bisacetamide |
| Abstract: | The differentiation agent hexamethylene bisacetamide (HMBA) increased expression of transforming growth factor ffi (TGFft) mRNA in HT29 colon carcinoma cells. The increase was evident after 24 h and was maintained at levels 4-5-fold the control levels for at least 5-13 days. No increase in expression of TGF02 or TGFo mRNA was observed. Both TGF0i and HMBA induced loss of expression of a cell surface malignancy marker on HT29 cells, and both decreased cell growth in serum-free medium. Exogenously applied TGF & mimicked the growth-arresting effect of HMBA on three surgically resected moderately differentiated colon carcinomas in serum-free primary culture. Both TGFft and HMBA increased the tumor growth fraction in a second group of three more aggressive colon carcinomas, while neither agent had any measurable growth-modulating activity on two other colon carcinomas. The induction of TGFft mRNA by HMBA along with the parallel biological effects of HMBA and exogenously applied TGF & on resected carcinomas and on HT29 cells suggest that the effects of HMBA on colon carcinoma cells may be mediated in part by induction of TGFft. © 1990, American Association for Cancer Research. All rights reserved. |
| Keywords: | human cell; tumor markers, biological; colonic neoplasms; cell differentiation; tumor cells, cultured; cell culture; messenger rna; rna, messenger; carcinoma; transforming growth factors; transforming growth factor beta1; colon carcinoma; cytochemistry; hexamethylenebisacetamide; acetamides; human; priority journal; article; support, u.s. gov't, p.h.s.; support, u.s. gov't, non-p.h.s. |
| Journal Title: | Cancer Research |
| Volume: | 50 |
| Issue: | 2 |
| ISSN: | 0008-5472 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 1990-01-15 |
| Start Page: | 261 |
| End Page: | 265 |
| Language: | English |
| PUBMED: | 2295065 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 27 January 2020 -- Source: Scopus |
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