| Abstract: |
Four human colon adenocarcinoma cell lines, SNU-C1, SNU-C4, SNU-C5, and NCI-H716, that are capable of proliferating autonomously in serum-free medium containing no added peptide growth factors were identified. All four cell lines show epidermal growth factor (EGF) receptors (EGFRs), express transforming growth factor α (TGF-α) messenger RNA, and release anti-TGF-α-immunoreactive molecules. The blocking anti-EGFR monoclonal antibody (mAb) 225 blocks autonomous proliferation of SNU-C1 and SNU-C4 cells. In both of these cell lines, the inhibitory effect of mAb 225 is reversible by the addition of EGF, TGF-α, or conditioned medium from any of the four cell lines. In contrast, autonomous proliferation of SNU-C5 and NCI-H716 cells is not inhibited by mAb 225 and is not affected by exogenous EGF, TGF-α, or conditioned medium. Together, these data confirm the previous finding that anti-EGFR antibodies can inhibit the proliferation of some carcinoma cell lines that coexpress TGF-α and EGFR. However, here it is shown that the mechanisms of autonomous proliferation of colon carcinoma cell lines are heterogeneous and not always sensitive to antibody disruption of TGF-α/ EGFR autocrine interactions. © 1992. |
| Keywords: |
epidermal growth factor; controlled study; human cell; cancer growth; polymerase chain reaction; adenocarcinoma; cell division; epidermal growth factor receptor; colonic neoplasms; receptor, epidermal growth factor; cancer cell culture; tumor cells, cultured; immunoreactivity; monoclonal antibody; antibodies, monoclonal; molecular sequence data; messenger rna; rna, messenger; base sequence; colon adenocarcinoma; receptor binding; transforming growth factor alpha; radioimmunoassay; radioligand assay; human; priority journal; article; support, u.s. gov't, p.h.s.; support, u.s. gov't, non-p.h.s.; radioreceptor assay
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