Increased cell size, structural complexity and migration of cancer cells acquiring fibroblast organelles by cell-projection pumping Journal Article
| Authors: | Zoellner, H.; Chami, B.; Kelly, E.; Moore, M. A. S. |
| Article Title: | Increased cell size, structural complexity and migration of cancer cells acquiring fibroblast organelles by cell-projection pumping |
| Abstract: | We recently described a hydrodynamic mechanism for cytoplasmic transfer between cells, termed cell-projection pumping (CPP). Earlier image analysis related altered SAOS-2 osteosarcoma cell morphology, to what we now recognize as CPP uptake of fibroblast cytoplasm. We here examine SAOS-2 phenotype following co-culture with human dermal fibroblasts (HDF) in which organelles were pre-labelled with a fluorescent lipophilic marker. Fluorescence activated cell sorting (FACS) analysis was performed of HDF and SAOS-2, cultured either alone or together. FACS forward scatter is proportionate to cell size, and increased for SAOS-2 with high levels of HDF fluorescence uptake (p < 0.004). FACS side scatter is proportionate to internal cell complexity, and increased in SAOS-2 with increasing uptake of HDF fluorescence (p < 0.004), consistent with uptake of HDF organelles. Scratch migration assays revealed that HDF migrated more quickly than SAOS-2 in both isolated cell culture, and following co-culture (p < 0.004). Notably, SAOS-2 with high levels of HDF labelling migrated faster compared with SAOS-2 with low HDF labelling (p < 0.008). A slight and unconvincing reduction in SAOS-2 proliferation was seen (p < 0.02). Similar results were obtained in single additional experiments with A673 and H312 cancer cells. Forward and side scatter results suggest organellar transfer by CPP increases cancer cell morphological diversity. This may contribute to histological pleomorphism relevant to cancer diagnosis and prognosis. Also, increased migration of sub-populations of cancer cells with high CPP organellar uptake, may contribute to invasion and metastasis in-vivo. We thus suggest relevance of CPP to cancer diagnosis and progression. © 2019 Zoellner et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| Keywords: | controlled study; human cell; cancer growth; comparative study; cancer diagnosis; cell proliferation; phenotype; cell division; cell structure; in vivo study; cancer therapy; quantitative analysis; cancer cell; cell migration; fibroblast; cell size; fluorescence activated cell sorting; cell invasion; cell transfer; coculture; human; article; cell migration assay; a-673 cell line; cell projection pumping; nci-h3122 cell line; saos-2 cell line |
| Journal Title: | PLoS ONE |
| Volume: | 14 |
| Issue: | 11 |
| ISSN: | 1932-6203 |
| Publisher: | Public Library of Science |
| Date Published: | 2019-11-07 |
| Start Page: | e0224800 |
| Language: | English |
| DOI: | 10.1371/journal.pone.0224800 |
| PUBMED: | 31697757 |
| PROVIDER: | scopus |
| PMCID: | PMC6837525 |
| DOI/URL: | |
| Notes: | Article -- Source: Scopus |
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