Aneuploidy affects proliferation and spontaneous immortalization in mammalian cells Journal Article
| Authors: | Williams, B. R.; Prabhu, V. R.; Hunter, K. E.; Glazier, C. M.; Whittaker, C. A.; Housman, D. E.; Amon, A. |
| Article Title: | Aneuploidy affects proliferation and spontaneous immortalization in mammalian cells |
| Abstract: | Aneuploidy, an incorrect number of chromosomes, is the leading cause of miscarriages and mental retardation in humans and is a hallmark of cancer. We examined the effects of aneuploidy on primary mouse cells by generating a series of cell lines that carry an extra copy of one of four mouse chromosomes. In all four trisomic lines, proliferation was impaired and metabolic properties were altered. Immortalization, the acquisition of the ability to proliferate indefinitely, was also affected by the presence of an additional copy of certain chromosomes. Our data indicate that aneuploidy decreases not only organismal but also cellular fitness and elicits traits that are shared between different aneuploid cells. |
| Keywords: | nonhuman; cell proliferation; animal cell; chromosome; mouse; metabolism; mammalia; animals; mice; cell cycle; gene expression; cell line; animal experiment; cell transformation, neoplastic; genetic engineering; genomic instability; fibroblasts; translocation, genetic; embryo, mammalian; cell size; glucose; fitness; rodent; gene dosage; health risk; cell metabolism; aneuploidy; cell aging; glutamine; culture media; mammal cell; metabolic networks and pathways; trisomy; cell immortalization; cell volume; serial passage |
| Journal Title: | Science |
| Volume: | 322 |
| Issue: | 5902 |
| ISSN: | 0036-8075 |
| Publisher: | American Association for the Advancement of Science |
| Date Published: | 2008-10-31 |
| Start Page: | 703 |
| End Page: | 709 |
| Language: | English |
| DOI: | 10.1126/science.1160058 |
| PUBMED: | 18974345 |
| PROVIDER: | scopus |
| PMCID: | PMC2701511 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 87" - "Export Date: 17 November 2011" - "CODEN: SCIEA" - "Source: Scopus" |
