Response to multiple radiation doses of fibroblasts over-expressing dominant negative Ku70 Journal Article
| Authors: | Urano, M.; Huang, Y.; He, F.; Minami, A.; Ling, C. C.; Li, G. C. |
| Article Title: | Response to multiple radiation doses of fibroblasts over-expressing dominant negative Ku70 |
| Abstract: | Purpose: To evaluate the response of cells over-expressing dominant negative (DN) Ku70 to single and multiple small radiation doses. Methods and Materials: Clones of fibroblasts over-expressing DNKu70, DNKu70-7, DNKu70-11, and parental Rat-1 cells were irradiated under oxic or hypoxic conditions with single or multiple doses. Cells were trypsinized 0 or 6 h after irradiation to determine surviving fraction (SF). Results: Oxic DNKu70-7 or -11 cells trypsinized 6 h after irradiation were 1.52 or 1.25 and 1.28 or 1.15 times more sensitive than oxic Rat-1 at SF of 0.5 and 0.1, respectively. Hypoxic DNKu70-7 or -11 cells trypsinized 6 h after irradiation were 1.44 or 1.70 and 1.33 or 1.51 times more sensitive than hypoxic Rat-1 at SF of 0.5 and 0.1, respectively. To the multiple doses, oxic and hypoxic DNKu70-7 or -11 cells were 1.35 or 1.37 and 2.23 or 4.61 times more sensitive than oxic and hypoxic Rat-1, respectively, resulting in very small oxygen enhancement ratios. Namely, enhancement caused by DNKu70 under hypoxia after multiple doses was greater than that under oxic conditions and greater than that after single dose. Conclusions: Over-expression of DNKu70 enhances cells' response to radiation given as a single dose and as multiple small doses. The enhancement after multiple doses was stronger under hypoxic than under oxic conditions. These results encourage the use of DNKu70 fragment in a gene-radiotherapy. © 2008 Elsevier Inc. All rights reserved. |
| Keywords: | controlled study; protein expression; treatment response; dna-binding proteins; nonhuman; radiation dose; linear models; animal cell; animals; dna damage; cell survival; dna repair; gene expression; radiotherapy; cell line; time factors; dose-response relationship, radiation; radiation dosage; molecular cloning; dosimetry; rat; irradiation; fibroblast; fibroblasts; cell hypoxia; rats; ku antigen; fractionation; trypsin; radiation tolerance; dna-activated protein kinase; gene-radiotherapy; ku70; oxygen enhancement; antigens, nuclear; dominant-negative (dn) ku70; pld repair; kurchatovium; surviving fraction; oxygen concentration |
| Journal Title: | International Journal of Radiation Oncology, Biology, Physics |
| Volume: | 71 |
| Issue: | 2 |
| ISSN: | 0360-3016 |
| Publisher: | Elsevier Inc. |
| Date Published: | 2008-06-01 |
| Start Page: | 533 |
| End Page: | 541 |
| Language: | English |
| DOI: | 10.1016/j.ijrobp.2007.12.061 |
| PUBMED: | 18374502 |
| PROVIDER: | scopus |
| PMCID: | PMC2723945 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 2" - "Export Date: 17 November 2011" - "CODEN: IOBPD" - "Source: Scopus" |
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