Mouse embryonic stem cells exhibit high levels of extrachromosomal homologous recombination in a chloramphenicol acetyltransferase assay system Journal Article
| Authors: | Jasin, M.; Liang, F. |
| Article Title: | Mouse embryonic stem cells exhibit high levels of extrachromosomal homologous recombination in a chloramphenicol acetyltransferase assay system |
| Abstract: | Mouse embryonic stem (ES) cells were compared to COS1 and CV1 cells for their ability to perform extrachromosomal homologous recombination. RSVCAT plasmid substrates consisting of overlapping chloramphenlcol acetyltransferase (CAT) gene fragments were transiently transfected into cells and extracts were assayed for CAT activity. Approximately 10% activity, relative to transfectlon with a complete CAT gene, was recovered for the recombination substrates In each of the cell lines tested. ES cells, therefore, as other cell lines, are capable of high levelsof extrachromosomal recombination. © 1991 Oxford University Press. |
| Keywords: | nonhuman; comparative study; animal cell; mouse; animal; mice; gene expression; embryo; cell line; transfection; assay; stem cell; genetic recombination; genetic transfection; recombination, genetic; stem cells; plasmids; biological assay; sequence homology, nucleic acid; embryo cell; blotting, southern; priority journal; article; chloramphenicol acetyltransferase; chloramphenicol o-acetyltransferase; extrachromosomal dna |
| Journal Title: | Nucleic Acids Research |
| Volume: | 19 |
| Issue: | 25 |
| ISSN: | 0305-1048 |
| Publisher: | Oxford University Press |
| Date Published: | 1991-12-25 |
| Start Page: | 7171 |
| End Page: | 7175 |
| Language: | English |
| DOI: | 10.1093/nar/19.25.7171 |
| PUBMED: | 1766877 |
| PROVIDER: | scopus |
| PMCID: | PMC332558 |
| DOI/URL: | |
| Notes: | Source: Scopus |
