Cdc7-Drf1 kinase links chromosome cohesion to the initiation of DNA replication in Xenopus egg extracts Journal Article
| Authors: | Takahashi, T. S.; Basu, A.; Bermudez, V.; Hurwitz, J.; Walter, J. C. |
| Article Title: | Cdc7-Drf1 kinase links chromosome cohesion to the initiation of DNA replication in Xenopus egg extracts |
| Abstract: | To establish functional cohesion between replicated sister chromatids, cohesin is recruited to chromatin before S phase. Cohesin is loaded onto chromosomes in the G1 phase by the Scc2-Scc4 complex, but little is known about how Scc2-Scc4 itself is recruited to chromatin. Using Xenopus egg extracts as a vertebrate model system, we showed previously that the chromatin association of Scc2 and cohesin is dependent on the prior establishment of prereplication complexes (pre-RCs) at origins of replication. Here, we report that Scc2-Scc4 exists in a stable complex with the Cdc7-Drf1 protein kinase (DDK), which is known to bind pre-RCs and activate them for DNA replication. Immunodepletion of DDK from Xenopus egg extracts impairs chromatin association of Scc2-Scc4, a defect that is reversed by wild-type, but not catalytically inactive DDK. A complex of Scc4 and the N terminus of Scc2 is sufficient for chromatin loading of Scc2-Scc4, but not for cohesin recruitment. These results show that DDK is required to tether Scc2-Scc4 to pre-RCs, and they underscore the intimate link between early steps in DNA replication and cohesion. © 2008 by Cold Spring Harbor Laboratory Press. |
| Keywords: | nonhuman; dna replication; protein function; mitosis; cohesin; animal cell; chromosome; animals; cell cycle proteins; chromosomal proteins, non-histone; oocytes; biological model; protein protein interaction; protein; protein stability; wild type; vertebrata; amino terminal sequence; chromatin; protein-serine-threonine kinases; nucleotide sequence; enzyme inactivation; immunoblotting; sister chromatid exchange; immunoassay; catalysis; enzyme binding; vertebrate; protein kinase; cell extracts; xenopus; oocyte development; xenopus laevis; cohesion; rabbits; xenopus proteins; cdc7-drf1; scc2-scc4; cdc7 drf1 protein kinase; protein scc2; protein scc4 |
| Journal Title: | Genes and Development |
| Volume: | 22 |
| Issue: | 14 |
| ISSN: | 0890-9369 |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Date Published: | 2008-07-15 |
| Start Page: | 1894 |
| End Page: | 1905 |
| Language: | English |
| DOI: | 10.1101/gad.1683308 |
| PUBMED: | 18628396 |
| PROVIDER: | scopus |
| PMCID: | PMC2492736 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 23" - "Export Date: 17 November 2011" - "CODEN: GEDEE" - "Molecular Sequence Numbers: GENBANK: NP_593137, TC260044, TC290672, EU747648;" - "Source: Scopus" |
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