Antiserum from a patient with cerebellar degeneration identifies a novel protein in purkinje cells, cortical neurons, and neuroectodermal tumors Journal Article
| Authors: | Darnell, R. B.; Furneaux, H. M.; Posner, J. B. |
| Article Title: | Antiserum from a patient with cerebellar degeneration identifies a novel protein in purkinje cells, cortical neurons, and neuroectodermal tumors |
| Abstract: | The serum and cerebrospinal fluid of a patient (NB) with subacute cerebellar degeneration were found to contain a novel antineuronal autoantibody (anti-Nb). Using this antibody, we have identified and characterized antigens present in a subset of neurons in the CNS and in some neuroectodermal tumor lines. Anti-Nb antibody bound to antigens of Mr 150, 120, and 65 kDa in Western blots using extracts of human cerebellar Purkinje cells or human cerebral cortical neurons. Immunohistochemistry demonstrated relatively specific binding of anti-Nb IgG to Purkinje cells in sections of human cerebellum and to some neocortical neurons, especially those in layer VI. Because of the association of cerebellar degeneration with occult malignancies, we screened a number of tumor cell lines for immunoreactivity to anti-Nb antibody; only tumor lines of neuroectodermal origin (melanoma, small-cell lung cancer, and neuroblastoma) expressed the Nb antigen. Anti-Nb antibody thus identifies neuronal and tumor cell antigens that appear to be unique in size and distribution of expression. Copyright ©1991 Society for Neuroscience. |
| Keywords: | immunohistochemistry; adult; controlled study; human tissue; case report; neoplasms; purkinje cell; purkinje cells; melanoma; lung neoplasms; cell line; neurons; blotting, western; brain; neuroblastoma; tumor cell line; cerebral cortex; autoantibody; molecular weight; autoantibodies; brain protein; carcinoma, small cell; immune sera; cerebellum degeneration; human; female; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; spinocerebellar degenerations |
| Journal Title: | The Journal of Neuroscience |
| Volume: | 11 |
| Issue: | 5 |
| ISSN: | 0270-6474 |
| Publisher: | Society for Neuroscience |
| Date Published: | 1991-05-01 |
| Start Page: | 1224 |
| End Page: | 1230 |
| Language: | English |
| PUBMED: | 1851215 |
| PROVIDER: | scopus |
| PMCID: | PMC6575333 |
| DOI: | 10.1523/JNEUROSCI.11-05-01224.1991 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 27 September 2019 -- Source: Scopus |
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