Isoform-specific transforming growth factor-β binding proteins with membrane attachments sensitive to phosphatidylinositol-specific phospholipase C Journal Article
| Authors: | Cheifetz, S.; Massagué, J. |
| Article Title: | Isoform-specific transforming growth factor-β binding proteins with membrane attachments sensitive to phosphatidylinositol-specific phospholipase C |
| Abstract: | We report the identification of cell surface glycoproteins that bind transforming growth factor-β (TGF-β) in an isoform-specific manner, and are distinct from TGF-β receptors I and II or the TGF-β binding proteoglycan betaglycan. The novel TGF-β binding proteins have been identified in various cell lines including fetal bovine heart endothelial cells and MG-63 human osteosarcoma cells. They include proteins of 90-100 and 180 kDa that preferentially bind TGF-β1 (K(D) 0.1-0.2 nM) and proteins of 60 and 140 kDa that preferentially bind TGF-β2 (K(D) 0.5-1 nM). The 180-kDa TGF-β1 binding protein and the 60- and 140-kDa TGF-β2 binding proteins can be released from the cell surface by treatment with phosphatidylinositol-specific phospholipase C, suggesting that these proteins are attached to the plasma membrane through a phosphatidylinositol anchor. The expression of these three proteins as well as their sensitivity to phosphatidylinositol-specific phospholipase C is cell line-dependent. The 90-100-kDa TGF-β1 binding proteins are components of a 190-kDa disulfide-linked complex. The structural properties of these proteins and their high affinity and selectivity for different TGF-β isoforms defines them as a novel class of cell surface TGF-β binding proteins. |
| Keywords: | nonhuman; animal cell; animal; transforming growth factor beta; cell line; endothelium cell; membrane glycoproteins; receptors, transforming growth factor beta; binding protein; cattle; fetus; molecular weight; isoprotein; disulfides; membrane binding; receptors, cell surface; binding, competitive; phosphoric diester hydrolases; proteoglycan; dithiothreitol; phospholipase c; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s. |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 266 |
| Issue: | 31 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 1991-11-05 |
| Start Page: | 20767 |
| End Page: | 20772 |
| Language: | English |
| PUBMED: | 1657936 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 27 September 2019 -- Source: Scopus |
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