No retroviremia or pathology in long-term follow-up of monkeys exposed to a murine amphotropic retrovirus Journal Article
| Authors: | Cornetta, K.; Morgan, R. A.; Gillio, A.; Sturm, S.; Baltrucki, L.; O'Reilly, R.; Anderson, W. F. |
| Article Title: | No retroviremia or pathology in long-term follow-up of monkeys exposed to a murine amphotropic retrovirus |
| Abstract: | Four monkeys were exposed to a retroviral vector and replication-competent murine amphotropic retrovirus in a bone marrow transplantation/gene transfer protocol (Kantoff et al., 1987). We have studied these animals 2 and 3 years post-transplantation and did not detect replicating virus in serum, peripheral blood mononuclear cells, or bone marrow cells. Amphotropic envelope sequences could not be detected in blood or bone marrow cells by Southern blotting or the polymerase chain reaction. Antibodies directed against the p30 and gp70 viral antigens were detected by Western blot and immunoprecipitation. The animals remain alive and well. Our findings suggest that primates can clear murine amphotropic retroviruses even when exposure occurs during a time of severe immunosuppression. |
| Keywords: | retrospective studies; genetics; molecular genetics; polymerase chain reaction; animal; bone marrow; retrospective study; physiology; animalia; genetic vectors; blotting, western; blood; immunology; molecular sequence data; leukocytes, mononuclear; genetic engineering; nucleotide sequence; recombinant proteins; recombinant protein; western blotting; murinae; base sequence; dna, viral; virus replication; monocyte; immune deficiency; immunocompromised host; microbiology; bone marrow transplantation; macaca; isolation and purification; retrovirus; retroviridae; southern blotting; virus dna; macaca fascicularis; viremia; primates; antibodies, viral; virus antibody; murine leukemia virus; adenosine deaminase; leukemia virus, murine; blotting, southern; dna vector; genes, env; unidentified retrovirus; article; envelope gene |
| Journal Title: | Human Gene Therapy |
| Volume: | 2 |
| Issue: | 3 |
| ISSN: | 1043-0342 |
| Publisher: | Mary Ann Liebert, Inc |
| Date Published: | 1991-10-01 |
| Start Page: | 215 |
| End Page: | 219 |
| Language: | English |
| DOI: | 10.1089/hum.1991.2.3-215 |
| PUBMED: | 1661171 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 27 September 2019 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work