Overexpression of TAR sequences renders cells resistant to human immunodeficiency virus replication Journal Article
| Authors: | Sullenger, B. A.; Gallardo, H. F.; Ungers, G. E.; Gilboa, E. |
| Article Title: | Overexpression of TAR sequences renders cells resistant to human immunodeficiency virus replication |
| Abstract: | Overexpression of TAR-containing sequences (TAR decoys) was used to render cells resistant to HIV replication. A chimeric tRNAmeti-TAR transcription unit contained in a double copy murine retroviral vector was used to express high levels of HIV-1 TAR-containing transcripts in CEM SS cells. Replication of HIV-1 was inhibited over 99% in cells expressing chimeric tRNA-TAR transcripts, but an amphotropic murine retrovirus replicated normally in these cells. Expression of TAR sequences in CEM SS cells had no adverse effects on cell viability, indicating that essential cellular factors are not being sequestered in these cells. TAR decoy RNA-mediated HIV inhibition may also be effective against natural HIV isolates in spite of their hypervariable nature, as suggested by the fact that replication of SIVmac was also inhibited in cells expressing HIV-1 TAR decoys. © 1990. |
| Keywords: | nonhuman; cell viability; gene expression; cell line; transcription, genetic; genetic vectors; molecular sequence data; murinae; base sequence; human immunodeficiency virus; virus replication; rna, viral; human immunodeficiency virus 1; hiv-1; virus cell interaction; hiv long terminal repeat; rna, transfer, met; oligonucleotide probes; unidentified retrovirus; human; priority journal; article; support, non-u.s. gov't |
| Journal Title: | Cell |
| Volume: | 63 |
| Issue: | 3 |
| ISSN: | 0092-8674 |
| Publisher: | Cell Press |
| Date Published: | 1990-11-02 |
| Start Page: | 601 |
| End Page: | 608 |
| Language: | English |
| DOI: | 10.1016/0092-8674(90)90455-n |
| PUBMED: | 2225067 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Source: Scopus |
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