A distinct kinase modulates the expression of IFN-inducible genes in human breast cancer cells Journal Article
| Authors: | Tiwari, R. K.; Osborne, M. P. |
| Article Title: | A distinct kinase modulates the expression of IFN-inducible genes in human breast cancer cells |
| Abstract: | The biological activity of interferons (IFNs) is presumed to be mediated through the induction of a number of IFN-inducible genes. IFN-mediated gene induction was examined in two human breast cancer cell lines, MCF-7 and BT-20. Both these cell lines were remarkably responsive to IFNs as a number of IFN inducible genes were rapidly induced. We examined the sensitivity of these genes towards 2-aminopurine (2-AP), a known inhibitor of double-stranded (ds) RNA dependent protein kinase. 2-AP has also been reported to inhibit the induction of IFN-β1 in response to dsRNA and the genes c-myc and c-fos in fibroblasts. In both MCF-7 and BT-20 cell lines, 2-AP selectively inhibited the IFN-induced gene responses. 2-AP did not affect levels of the oncogene, HER-2/neu. Tamoxifen (TAM), an antiestrogenic drug, which is known to inhibit the activity of protein kinase C at high concentrations, did not affect IFN-mediated gene induction. Our data is consistent with the concept that the 2-AP sensitive kinase is primarily associated with the IFN-induced gene systems and that positive and negative growth regulating stimulii in breast cancer may require the participation of distinct kinases. © 1991. |
| Keywords: | controlled study; human cell; interferon; breast cancer; gene expression; estrogens; cancer cell culture; tumor cells, cultured; breast neoplasms; gene expression regulation, neoplastic; tamoxifen; receptor, erbb-2; phosphotransferases; mcf-7 cells; neoplasms, hormone-dependent; interferon type i; oncogene proteins, viral; her-2/neu; 2 aminopurine; 2-aminopurine; interferons; human; female; priority journal; article; mink cell focus-forming virus; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; bt-20 cells; ifn-inducible genes; mrna analyses |
| Journal Title: | Cancer Letters |
| Volume: | 59 |
| Issue: | 1 |
| ISSN: | 0304-3835 |
| Publisher: | Elsevier Ireland Ltd. |
| Date Published: | 1991-07-26 |
| Start Page: | 31 |
| End Page: | 36 |
| Language: | English |
| DOI: | 10.1016/0304-3835(91)90132-2 |
| PUBMED: | 1715233 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 27 September 2019 -- Source: Scopus |
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