Augmentation of cytotoxicity using combinations of interferons (types I and II), tumor necrosis factor-α, and tamoxifen in MCF-7 cells Journal Article
| Authors: | Tiwari, R. K.; Wong, G. Y.; Liu, J.; Miller, D.; Osborne, M. P. |
| Article Title: | Augmentation of cytotoxicity using combinations of interferons (types I and II), tumor necrosis factor-α, and tamoxifen in MCF-7 cells |
| Abstract: | The cytotoxic effect of a combination of interferons (type I and II) and tumor necrosis factor (TNF), with an antiestrogenic drug, tamoxifen (TAM), was investigated in the estrogen receptor positive human breast carcinoma cell line, MCF-7. Cytotoxicity was measured by the MTT assay. In an attempt to define the molecular basis for the interaction between the interferons (IFNs) and TNF or any one of the cytokines with TAM, the induction characteristics of a number of IFN-induced mRNAs in response to IFNs, TNF, and TAM were studied. We observed an augmentation of the cytotoxic effect of TNF when it was combined with TAM. There appears to be an overlap in signalling mechanisms of IFNs and TNF as two of the IFN-inducible genes, 1-8 and 6-16 are also induced by TNF. mRNA 1-8 was induced by both IFN-α (type I) and IFN-γ (type II). We conclude that TNF potentiates the cytotoxic effects of TAM in MCF-7 cells and that the three cytokines IFN-α, IFN-γ, and TNF share some pathways that lead to specific induction of some cytokine responsive genes. © 1991. |
| Keywords: | signal transduction; controlled study; human cell; interferon; drug potentiation; alpha interferon; antineoplastic combined chemotherapy protocols; cytotoxicity; tumor cells, cultured; breast neoplasms; drug synergism; gene expression regulation, neoplastic; cytokine; cytokines; regulatory mechanism; tumor necrosis factor alpha; gamma interferon; messenger rna; breast carcinoma; tamoxifen; cell strain mcf 7; mcf-7 cells; tumor necrosis factor; tumor necrosis; interferon type i; synergism; interferon type ii; interferons; human; female; priority journal; article; mink cell focus-forming virus; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; inducible mrnas |
| Journal Title: | Cancer Letters |
| Volume: | 61 |
| Issue: | 1 |
| ISSN: | 0304-3835 |
| Publisher: | Elsevier Ireland Ltd. |
| Date Published: | 1991-12-09 |
| Start Page: | 45 |
| End Page: | 52 |
| Language: | English |
| DOI: | 10.1016/0304-3835(91)90075-s |
| PUBMED: | 1764697 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 27 September 2019 -- Source: Scopus |
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