E-cadherin gene alterations in gastric cancers in different ethnic populations Journal Article
| Authors: | Steinberg, M. L.; Hwang, B. J.; Tang, L.; Shah, M. A. |
| Article Title: | E-cadherin gene alterations in gastric cancers in different ethnic populations |
| Abstract: | Introduction: A retrospective study of nucleotide sequence alterations in exons 7-9 of the E-cadherin gene and expression of E-cadherin and β-catenin in gastric tumors from African American, Asian, Causcasian and Hispanic patients was carried out to determine differences potentially related to race/ethnicity in these groups. Methods: Paraffin-embedded tissue sections archived at the Memorial Sloan Kettering Cancer Center were used for immunohisto- chemical staining of sections for membranous E-cadherin protein and nuclear localization of β-catenin. DNA from tumor tissue extracted from the paraffin sections was used as template for amplification of the E-cadherin gene exonic regions. Results: Sequence analyses of the high-frequency mutation region along E-cadherin exons 7-9 revealed a number sequence alterations in the patient group as a whole, mostly within exon 8. The alterations were mainly single nucleotide insertions, buta palindromic duplication of exon 8 in a Caucasian patient and several extragenic insertions in a Hispanic and an African American patient were also found. Nuclear localization of β-catenin was correlated with inactivating sequence alterations in three patients. Conclusions: Exon 8 was found to display the most extensive alterations in all the groups studied. As a group, the most extensive sequence alterations were found in tumors from Caucasian patients. A finding of potential significance as a biomarker related to ethnicity was a C insertion at nt 76,598 found independently in two African American patients. |
| Keywords: | immunohistochemistry; controlled study; human tissue; retrospective studies; gene mutation; gene sequence; promoter region; sequence analysis; exon; genetics; mutation; exons; polymerase chain reaction; protein localization; biological marker; gene amplification; gene expression; tumor markers, biological; gene frequency; retrospective study; uvomorulin; tumor marker; tissue section; enzyme immunoassay; immunoenzyme techniques; cancer center; dna; nucleotide sequence; gene duplication; stomach cancer; dna sequence; gene insertion; ethnic difference; beta catenin; cadherin; cadherins; race difference; african americans; european continental ancestry group; stomach neoplasms; ethnicity; african american; stomach tumor; caucasian; paraffin; hispanic; asian american; sequence analysis, dna; hispanic americans; ethnology; asian; asian americans; gene insertion sequence; e cadherin alterations; ethnic biomarkers; gastric tumors; palindromic dna |
| Journal Title: | Ethnicity & Disease |
| Volume: | 18 |
| Issue: | 2 SUPPL. 2 |
| ISSN: | 1049-510X |
| Publisher: | Int Soc Hypertension Blacks Ishib |
| Date Published: | 2008-03-01 |
| Start Page: | S2-70 |
| End Page: | S2-74 |
| Language: | English |
| PUBMED: | 18646324 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 5" - "Export Date: 17 November 2011" - "CODEN: ETDIE" - "Molecular Sequence Numbers: GENBANK: DQ090940;" - "Source: Scopus" |
