Design and production of Newcastle disease virus for intratumoral immunomodulation Journal Article


Authors: Vijayakumar, G.; Zamarin, D.
Article Title: Design and production of Newcastle disease virus for intratumoral immunomodulation
Abstract: Newcastle disease virus (NDV) is an avian paramyxovirus that has been extensively studied as an oncolytic agent, in addition to being an economically important pathogen in the poultry industry. The establishment of a reverse genetics system for this virus has enabled the development of genetically modified recombinant NDV viruses with improved oncolytic and immunotherapeutic properties. In this chapter, we describe the materials and methods involved in the in vitro cloning and rescue of NDV expressing murine 4-1BBL as well as the in vivo evaluation of NDV expressing 4-1BBL in a B16-F10 murine melanoma model. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.
Keywords: immunotherapy; newcastle disease virus; reverse genetics; oncolytic vector; virus rescue
Journal Title: Methods in Molecular Biology
Volume: 2058
ISSN: 1064-3745
Publisher: Humana Press Inc  
Date Published: 2020-01-01
Start Page: 133
End Page: 154
Language: English
DOI: 10.1007/978-1-4939-9794-7_9
PUBMED: 31486036
PROVIDER: scopus
DOI/URL:
Notes: Chapter 9 in "Oncolytic Viruses" (ISBN: 978-1-4939-9793-0) -- Source: Scopus
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MSK Authors
  1. Dmitriy Zamarin
    65 Zamarin