Therapeutic effects of a fusogenic newcastle disease virus in treating head and neck cancer Journal Article
| Authors: | Li, P.; Chen, C. H.; Li, S.; Givi, B.; Yu, Z.; Zamarin, D.; Palese, P.; Fong, Y.; Wong, R. J. |
| Article Title: | Therapeutic effects of a fusogenic newcastle disease virus in treating head and neck cancer |
| Abstract: | Background Newcastle disease virus (NDV) is a paramyxovirus that is pathogenic in birds but causes only mild flulike symptoms in human beings. NDV(F3aa)-GFP is a genetically modified, fusogenic NDV. We assessed the utility of NDV(F3aa)-GFP in treating head and neck squamous cell carcinoma. Methods and Results At a multiplicity of infection (MOI) of 1, NDV(F3aa)-GFP infection of 3 cell lines supported strong GFP expression by 36 hours. Four cell lines were highly sensitivite to viral cytotoxicity, with >75% of cells lysed by day 6 at MOI 0.1, and 2 other cell lines were partially susceptible. Murine SCC25 flank tumors exhibited robust GFP expression after a single intratumoral viral injection and showed near-complete tumor regression over 34 days. There were no adverse effects attributable to therapy. Conclusions We demonstrate that a fusogenic NDV exerts potent oncolytic effects against human head and neck cancer and support its continued investigation for clinical application. © 2010 Wiley Periodicals, Inc. |
| Keywords: | controlled study; human cell; squamous cell carcinoma; carcinoma, squamous cell; nonhuman; mouse; animals; mice; gene expression; animal experiment; animal model; cancer cell culture; cytotoxicity; tumor regression; cell line, tumor; mice, nude; head and neck cancer; head and neck neoplasms; oncolytic virotherapy; green fluorescent proteins; virus infection; neoplasm transplantation; virus strain; head and neck carcinoma; newcastle disease virus; newcastle disease paramyxovirus; viral therapy; oncolysis |
| Journal Title: | Head & Neck |
| Volume: | 33 |
| Issue: | 10 |
| ISSN: | 1043-3074 |
| Publisher: | John Wiley & Sons, Inc. |
| Date Published: | 2011-10-01 |
| Start Page: | 1394 |
| End Page: | 1399 |
| Language: | English |
| DOI: | 10.1002/hed.21609 |
| PROVIDER: | scopus |
| PUBMED: | 21928411 |
| PMCID: | PMC3116983 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 2 November 2011" - "CODEN: HEANE" - "Source: Scopus" |
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